Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1790819..1791650 Replicon chromosome
Accession NZ_CP095125
Organism Escherichia coli strain Iso00270

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag MU416_RS08495 Protein ID WP_000854814.1
Coordinates 1790819..1791193 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag MU416_RS08500 Protein ID WP_001285585.1
Coordinates 1791282..1791650 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU416_RS08455 (1786224) 1786224..1787444 + 1221 WP_000343760.1 ISL3-like element ISKox3 family transposase -
MU416_RS08460 (1787501) 1787501..1787974 + 474 WP_032224276.1 DNA gyrase inhibitor SbmC -
MU416_RS08465 (1788171) 1788171..1789229 + 1059 WP_001200891.1 FUSC family protein -
MU416_RS08470 (1789401) 1789401..1789730 + 330 WP_000450409.1 DUF496 family protein -
MU416_RS08475 (1789831) 1789831..1789965 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
MU416_RS08480 (1790085) 1790085..1790213 + 129 Protein_1661 transposase domain-containing protein -
MU416_RS08485 (1790502) 1790502..1790582 - 81 Protein_1662 hypothetical protein -
MU416_RS08490 (1790628) 1790628..1790822 - 195 WP_000988600.1 DUF5983 family protein -
MU416_RS08495 (1790819) 1790819..1791193 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
MU416_RS08500 (1791282) 1791282..1791650 - 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
MU416_RS08505 (1791724) 1791724..1791945 - 222 WP_000692323.1 DUF987 domain-containing protein -
MU416_RS08510 (1792008) 1792008..1792484 - 477 WP_001186726.1 RadC family protein -
MU416_RS08515 (1792500) 1792500..1792979 - 480 WP_000860087.1 antirestriction protein -
MU416_RS08520 (1793061) 1793061..1793879 - 819 WP_001234629.1 DUF932 domain-containing protein -
MU416_RS08525 (1793979) 1793979..1794212 - 234 WP_001119729.1 DUF905 family protein -
MU416_RS08530 (1794291) 1794291..1794745 - 455 Protein_1671 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1786224..1787444 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T241428 WP_000854814.1 NZ_CP095125:c1791193-1790819 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T241428 NZ_LT992465:c723313-723206 [Staphylococcus aureus]
GTGATATCTATTGCAAATGCATTACATTTAATGTTAAGTTTCGGTATGTTTATCGTCACTTTCATTGGTGTAGTAGTCGC
AATAATTAATTTAAACAATAAAAAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT241428 WP_001285585.1 NZ_CP095125:c1791650-1791282 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT241428 NZ_LT992465:723116-723153 [Staphylococcus aureus]
AACATGTCGCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References