Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 652494..653221 Replicon chromosome
Accession NZ_CP095125
Organism Escherichia coli strain Iso00270

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag MU416_RS03200 Protein ID WP_000550189.1
Coordinates 652494..652808 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MU416_RS03205 Protein ID WP_000560266.1
Coordinates 652805..653221 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU416_RS03180 (648661) 648661..649647 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
MU416_RS03185 (649726) 649726..650409 - 684 WP_001183043.1 vancomycin high temperature exclusion protein -
MU416_RS03190 (650486) 650486..650989 - 504 WP_001295542.1 M48 family metallopeptidase -
MU416_RS03195 (651074) 651074..652210 + 1137 WP_000018681.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
MU416_RS03200 (652494) 652494..652808 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
MU416_RS03205 (652805) 652805..653221 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MU416_RS03210 (653266) 653266..655284 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
MU416_RS03215 (655710) 655710..658061 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T241424 WP_000550189.1 NZ_CP095125:652494-652808 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T241424 NZ_LT992464:1774902-1775090 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT241424 WP_000560266.1 NZ_CP095125:652805-653221 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT241424 NZ_LT992464:1774614-1774874 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References