Detailed information of TA system TA024137

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 245935..246962
• Replicon: chromosome
• Accession: NC_012968
• Organism: Methylotenera mobilis JLW8

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: MMOL_RS01140
• Protein ID: WP_012777590.1
• Coordinates: 245935..246501 (-)
• Length: 189 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: C6WSP2
• Locus tag: MMOL_RS01145
• Protein ID: WP_012777591.1
• Coordinates: 246507..246962 (-)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMOL_RS01120	241214..241558	-	345	WP_012777586.1	helix-turn-helix transcriptional regulator	-
MMOL_RS01125	241677..241928	+	252	WP_041928509.1	hypothetical protein	-
MMOL_RS01130	241940..242479	+	540	WP_012777588.1	DUF4755 domain-containing protein	-
MMOL_RS01135	242524..245745	-	3222	WP_012777589.1	type I restriction endonuclease subunit R	-
MMOL_RS01140	245935..246501	-	567	WP_012777590.1	PIN domain-containing protein	Toxin
MMOL_RS01145	246507..246962	-	456	WP_012777591.1	helix-turn-helix domain-containing protein	Antitoxin
MMOL_RS01150	247042..248004	-	963	WP_012777592.1	DUF4868 domain-containing protein	-
MMOL_RS01155	248004..248585	-	582	WP_012777593.1	hypothetical protein	-
MMOL_RS01160	248582..250576	-	1995	WP_012777594.1	ATP-binding protein	-
MMOL_RS01165	250569..251762	-	1194	WP_012777595.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	239631..262202	22571

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• SinI-like, DNA-binding domain (81-127)

Sequences

Toxin

Protein

• Length: 189 a.a.
• Molecular weight: 21493.85 Da
• Isoelectric Point: 4.7560

>T24137 WP_012777590.1 NC_012968:c246501-245935 [Methylotenera mobilis JLW8]
MARFAVLFDACVLYPAALRDSLMRLANCDLFKAYWTDAIHEEWINALVREDKYPLEVLERVRNLMDSHVPDAKVEGYECL
IEGLALPDKDDRHVLAAAIKCKADAIVTFNQKDFPADYLQTFQIEIIHPDDFLYYQIDMAPAICCKAFKDQRSALKNPPM
SVDEFLTCLQKQQLPQTVSSLKNYAGLI

Nucleotide

• Length: 567 bp

>T24137 NC_012968:c246501-245935 [Methylotenera mobilis JLW8]
ATGGCACGTTTTGCCGTTTTGTTTGACGCATGCGTGCTTTACCCTGCAGCTCTGCGCGACAGCCTAATGCGCTTAGCAAA
CTGTGATTTGTTTAAAGCCTATTGGACAGATGCGATACATGAAGAATGGATCAATGCTCTAGTGCGCGAAGATAAATACC
CGCTAGAGGTACTTGAGCGTGTGCGCAACTTGATGGACAGTCATGTGCCTGATGCAAAAGTAGAAGGCTATGAATGTTTG
ATTGAAGGTTTAGCCCTACCAGATAAAGATGATAGGCATGTGCTTGCTGCCGCCATAAAGTGCAAGGCAGATGCGATTGT
GACATTTAATCAAAAGGATTTTCCTGCTGATTATTTACAAACTTTTCAGATAGAAATCATCCACCCAGATGATTTTCTTT
ATTATCAAATAGACATGGCACCTGCGATTTGTTGTAAAGCTTTTAAAGACCAGCGCAGTGCTTTGAAAAATCCGCCTATG
TCTGTGGATGAATTTTTAACTTGTCTGCAAAAGCAACAGTTACCACAAACGGTTTCATCACTAAAAAATTATGCGGGCTT
GATTTAG

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 16493.07 Da
• Isoelectric Point: 7.0747

>AT24137 WP_012777591.1 NC_012968:c246962-246507 [Methylotenera mobilis JLW8]
MNAMQNVVHLPTKPEIEAAKQSSRMLSKYADADRVQLTLRANNGAAEDLVLPGQIMQLLLDIVSEMAKGNAISLVPIHHE
LSTQEAANLLNVSRPHLVSLLEKGELPHRKVGAHRRVLASDVLAYKESLLAKRNAALDELTAISQELGMGY

Nucleotide

• Length: 456 bp

>AT24137 NC_012968:c246962-246507 [Methylotenera mobilis JLW8]
ATGAACGCGATGCAAAATGTAGTGCATTTACCCACAAAGCCCGAGATTGAAGCTGCTAAACAATCTAGCCGTATGTTGAG
TAAATATGCGGATGCAGACAGGGTACAACTAACTTTACGTGCCAATAATGGCGCAGCTGAAGATTTGGTGTTGCCTGGGC
AAATTATGCAATTGCTGTTAGATATTGTCTCGGAAATGGCAAAGGGCAATGCGATTAGCTTGGTTCCAATTCACCATGAG
CTCAGTACACAAGAAGCAGCTAATTTACTGAATGTTAGCCGTCCGCATTTGGTGAGCTTATTAGAAAAAGGCGAACTACC
TCATCGTAAAGTAGGTGCCCATCGCCGTGTGTTAGCAAGCGATGTGTTGGCTTATAAAGAATCACTGTTGGCTAAGCGCA
ATGCAGCCCTTGATGAACTAACGGCTATATCGCAAGAGTTAGGTATGGGGTATTAA

Structures

Antitoxin

• AlphaFold DB: C6WSP2