Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2969854..2970502 Replicon chromosome
Accession NZ_CP095020
Organism Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag MUW65_RS14005 Protein ID WP_003899414.1
Coordinates 2969854..2970177 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag MUW65_RS14010 Protein ID WP_003899415.1
Coordinates 2970257..2970502 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW65_RS13975 (MUW65_13975) 2965177..2966175 + 999 WP_003900538.1 tyrosine-type recombinase/integrase -
MUW65_RS13980 (MUW65_13980) 2966189..2966653 + 465 WP_244702860.1 hypothetical protein -
MUW65_RS13985 (MUW65_13985) 2966641..2966892 + 252 WP_003908028.1 hypothetical protein -
MUW65_RS13990 (MUW65_13990) 2967063..2968502 - 1440 WP_003901443.1 phage major capsid protein -
MUW65_RS13995 (MUW65_13995) 2968510..2969043 - 534 WP_003899412.1 HK97 family phage prohead protease -
MUW65_RS14000 (MUW65_14000) 2969196..2969687 - 492 WP_003900541.1 phage terminase small subunit P27 family -
MUW65_RS14005 (MUW65_14005) 2969854..2970177 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
MUW65_RS14010 (MUW65_14010) 2970257..2970502 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
MUW65_RS14015 (MUW65_14015) 2970499..2971926 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
MUW65_RS14020 (MUW65_14020) 2971928..2972320 - 393 WP_003899417.1 DUF2742 domain-containing protein -
MUW65_RS14025 (MUW65_14025) 2972317..2972577 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
MUW65_RS14030 (MUW65_14030) 2972594..2972956 - 363 WP_003900543.1 hypothetical protein -
MUW65_RS14035 (MUW65_14035) 2972959..2974086 - 1128 WP_003899420.1 site-specific integrase -
MUW65_RS14040 (MUW65_14040) 2974231..2974458 - 228 WP_003899421.1 hypothetical protein -
MUW65_RS14045 (MUW65_14045) 2974455..2974844 - 390 WP_003899422.1 hypothetical protein -
MUW65_RS14050 (MUW65_14050) 2974750..2975022 + 273 WP_003900544.1 hypothetical protein -
MUW65_RS14055 (MUW65_14055) 2975121..2975354 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2965177..2974086 8909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T240997 WP_003899414.1 NZ_CP095020:c2970177-2969854 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T240997 NZ_LT903847:c4738991-4738677 [Escherichia coli O127:H6]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT240997 WP_003899415.1 NZ_CP095020:c2970502-2970257 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT240997 NZ_LT903847:c4739227-4738994 [Escherichia coli O127:H6]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References