Detailed information of TA system TA240861

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2151378..2151603
• Replicon: chromosome
• Accession: NZ_CP094987
• Organism: Shigella flexneri strain 3160_NCHU22

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: MT485_RS10715
• Protein ID: WP_000813254.1
• Coordinates: 2151448..2151603 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2151378..2151436 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT485_RS10675	2147376..2147545	+	170	Protein_2069	hypothetical protein	-
MT485_RS10680	2147691..2148437	+	747	WP_000788996.1	ATP-binding protein	-
MT485_RS10685	2148452..2148874	+	423	WP_001118168.1	DUF977 family protein	-
MT485_RS10690	2148932..2149288	+	357	WP_005048249.1	hypothetical protein	-
MT485_RS10695	2149381..2149599	+	219	WP_000256998.1	DUF4014 family protein	-
MT485_RS10700	2149601..2149966	+	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
MT485_RS10705	2149963..2150628	+	666	WP_000208062.1	hypothetical protein	-
MT485_RS10710	2150628..2150993	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	2151378..2151436	-	59	-	-	Antitoxin
MT485_RS10715	2151448..2151603	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
MT485_RS10725	2152940..2153539	+	600	WP_000940329.1	DUF1367 family protein	-
MT485_RS10730	2153539..2153829	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
MT485_RS10735	2153826..2154380	+	555	WP_000640143.1	DUF1133 family protein	-
MT485_RS10740	2154533..2154706	+	174	WP_000504450.1	hypothetical protein	-
MT485_RS10745	2154768..2155924	+	1157	WP_094081550.1	IS3-like element IS600 family transposase	-
MT485_RS10750	2155917..2156477	+	561	WP_072075205.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	2143461..2205335	61874

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T240861 WP_000813254.1 NZ_CP094987:2151448-2151603 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T240861 NZ_LT838196:c4078395-4078108 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT240861 NZ_CP094987:c2151436-2151378 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin