Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7998..8641 Replicon plasmid pKP4831-3
Accession NZ_CP094454
Organism Klebsiella pneumoniae subsp. rhinoscleromatis strain KP4831

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag MRR30_RS29840 Protein ID WP_001044770.1
Coordinates 8225..8641 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag MRR30_RS29835 Protein ID WP_001261282.1
Coordinates 7998..8228 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRR30_RS29800 (MRR30_29800) 3221..4000 - 780 WP_013214009.1 site-specific integrase -
MRR30_RS29805 (MRR30_29805) 4184..5188 - 1005 WP_011977814.1 hypothetical protein -
MRR30_RS29810 (MRR30_29810) 5218..5421 - 204 WP_011977813.1 hypothetical protein -
MRR30_RS29815 (MRR30_29815) 5467..5988 - 522 WP_013214008.1 hypothetical protein -
MRR30_RS29820 (MRR30_29820) 6046..6438 - 393 WP_011977811.1 hypothetical protein -
MRR30_RS29825 (MRR30_29825) 6475..7419 - 945 WP_011977810.1 hypothetical protein -
MRR30_RS29830 (MRR30_29830) 7580..8041 - 462 WP_014343465.1 hypothetical protein -
MRR30_RS29835 (MRR30_29835) 7998..8228 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MRR30_RS29840 (MRR30_29840) 8225..8641 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
MRR30_RS29845 (MRR30_29845) 8715..10277 + 1563 WP_004206609.1 AAA family ATPase -
MRR30_RS29850 (MRR30_29850) 10262..11284 + 1023 WP_000361404.1 helicase UvrD -
MRR30_RS29855 (MRR30_29855) 11540..12237 + 698 WP_223174630.1 IS1 family transposase -
MRR30_RS29860 (MRR30_29860) 12253..12654 + 402 Protein_16 conjugal transfer pilus-stabilizing protein TraP -
MRR30_RS29865 (MRR30_29865) 12641..12838 + 198 WP_001324648.1 conjugal transfer protein TrbD -
MRR30_RS29870 (MRR30_29870) 12850..13101 + 252 WP_001038341.1 conjugal transfer protein TrbG -
MRR30_RS29875 (MRR30_29875) 13098..13613 + 516 WP_062955122.1 type IV conjugative transfer system lipoprotein TraV -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..45644 45644


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T239857 WP_001044770.1 NZ_CP094454:8225-8641 [Klebsiella pneumoniae subsp. rhinoscleromatis]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T239857 NZ_LS483363:1335085-1335930 [Streptococcus dysgalactiae subsp. equisimilis]
ATGAACAAAGCTAAATTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGTTTAACCTTTAATTCCGTGATGACCTA
TTACTTCCTAGAAGTCATTTTGAAAAAATTAAGTCAAAGTTCTTATTCAAATCACTATATCTTCAAAGGAGGATTCCTAC
TGTCGAATGTCATCGGAGTTGAATCTCGTAGCACTGTTGATATTGACTTTTTATTCCATCAAATAACACTCTCAGAAGAA
ACTGTTAAACAGCAACTCAAGGAAATTTTAGCAGATTCCGAAGAAGGTATATCTTTTGTGATTCAATCAATCACAACTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACAATTTCGTGCCAACTTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCTCAGCCGATAACATACGACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAAAAACTCCAAACCATCTATTCACGAAACTTCTTGAACAGCAGAAG
CAAAGACTTTTACGATGTTTATATTCTTTCAAAATTGAAGAAAAAAGATATTGATTTCAATCAGTTAAAAAATGCTTGCC
AAAGAACTTTTTCCTATCGCGAAACTGAGCTCGATTTCGAAAAAATTATTGAACTACTCGAGAGATTCAAATCTGATCCT
ACTCAGAATCAACAATGGCAAAATTATTCAAAAAAATATAGTTATACAAAAGGAATTTCACTTGCAAATGTGCTTGATGA
GATGATTAGTCTTATTACAATTATTATTTCTACTGACTCTGATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT239857 WP_001261282.1 NZ_CP094454:7998-8228 [Klebsiella pneumoniae subsp. rhinoscleromatis]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT239857 NZ_LS483363:1334498-1335088 [Streptococcus dysgalactiae subsp. equisimilis]
ATGTCAAAAAAAGAGATTCTACTCGATTTTATAGAAAAGAACAATGGCATTGTCACAAACAAAGATTGTAAAGCACTAGG
TATTCCAACAATATATCTGACAAGACTAGAGAAAGAAGGCATTATCTTCCGAGTTGAAAAAGGTATTTTCCTAACCCAAA
ACGGAGACTATGATGAATACTACTTCTTTCAATATCGTTTTCCTAAGGCTATTTTCTCTTATATTTCGGCACTGTATCTA
CAACAGTTTACAGATGAAATTCCACAATATTTTGATGTGACAGTTCCTAGAGGCTACCGCTTTAATACTCCTCCAGCTAA
TCTAAACATTCACTTTGTCTCTAAAGAATACAGCGAGCTGGGAATGACTACTGTACCTACTCCTATGGGAAACAACGTAA
GGGTATATGATTTTGAACGTATTATTTGTGATTTTGTAATACATCGAGAAAAGATTGATTCCGAACTCTTTGTCAAGACA
CTTCAATCTTATGGGAACTATCCTAAAAAAAATCTAGCAAAACTCTATGAATATGCGACAAAAATGAATACTTTAGAAAA
AGTTAAACAAACTCTGGAGGTTCTAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References