Detailed information of TA system TA239527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 76578..77221
• Replicon: plasmid unnamed6
• Accession: NZ_CP094307
• Organism: Edwardsiella anguillarum strain R18-14-13

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MQ091_RS20210
• Protein ID: WP_283292435.1
• Coordinates: 76578..76994 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6C0NE67
• Locus tag: MQ091_RS20215
• Protein ID: WP_008322219.1
• Coordinates: 76991..77221 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQ091_RS20190 (MQ091_20165)	72198..72998	+	801	WP_283292457.1	IS21-like element helper ATPase IstB	-
MQ091_RS20195 (MQ091_20170)	73055..73774	+	720	Protein_78	IS3 family transposase	-
MQ091_RS20200 (MQ091_20175)	73935..74957	-	1023	WP_283292440.1	DNA helicase UvrD	-
MQ091_RS20205 (MQ091_20180)	74942..76504	-	1563	WP_283292438.1	AAA family ATPase	-
MQ091_RS20210 (MQ091_20185)	76578..76994	-	417	WP_283292435.1	type II toxin-antitoxin system VapC family toxin	Toxin
MQ091_RS20215 (MQ091_20190)	76991..77221	-	231	WP_008322219.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MQ091_RS20220 (MQ091_20195)	77537..79108	+	1572	WP_283292432.1	AAA family ATPase	-
MQ091_RS20225 (MQ091_20200)	79157..80026	-	870	WP_068872224.1	hypothetical protein	-
MQ091_RS20230 (MQ091_20205)	80028..81116	-	1089	WP_283292455.1	transcriptional repressor PifC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papC / papD	1..91492	91492

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15132.48 Da
• Isoelectric Point: 7.3236

>T239527 WP_283292435.1 NZ_CP094307:c76994-76578 [Edwardsiella anguillarum]
VNKTYMLDTNICSFIMREQPDVVLKHLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKLSLRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T239527 NZ_LR898868:c3579176-3578778 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8736.84 Da
• Isoelectric Point: 4.4692

>AT239527 WP_008322219.1 NZ_CP094307:c77221-76991 [Edwardsiella anguillarum]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFNL

Nucleotide

• Length: 231 bp

>AT239527 NZ_LR898868:c3579472-3579179 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A6C0NE67