Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4068964..4069514 Replicon chromosome
Accession NZ_CP094269
Organism Salmonella enterica subsp. enterica serovar Enteritidis strain JL228

Toxin (Protein)


Gene name ccdB Uniprot ID M7RJ32
Locus tag MPK88_RS20000 Protein ID WP_001199743.1
Coordinates 4068964..4069272 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID M7RP97
Locus tag MPK88_RS20005 Protein ID WP_001118105.1
Coordinates 4069275..4069514 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPK88_RS19965 (4064128) 4064128..4064457 - 330 WP_280165780.1 fimbria/pilus outer membrane usher protein -
MPK88_RS19970 (4064520) 4064520..4064750 - 231 WP_280164115.1 fimbria/pilus outer membrane usher protein -
MPK88_RS19975 (4064821) 4064821..4064970 - 150 WP_280164116.1 fimbria/pilus outer membrane usher protein -
MPK88_RS19980 (4066404) 4066404..4066520 - 117 WP_280164117.1 CD15/CS22/SEF14 family fimbrial major subunit -
MPK88_RS19985 (4066639) 4066639..4066899 - 261 Protein_3906 CD15/CS22/SEF14 family fimbrial major subunit -
MPK88_RS19990 (4067254) 4067254..4068387 + 1134 Protein_3907 IS3 family transposase -
MPK88_RS19995 (4068419) 4068419..4068558 - 140 Protein_3908 Arm DNA-binding domain-containing protein -
MPK88_RS20000 (4068964) 4068964..4069272 - 309 WP_001199743.1 CcdB family protein Toxin
MPK88_RS20005 (4069275) 4069275..4069514 - 240 WP_001118105.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
MPK88_RS20010 (4069623) 4069623..4069871 - 249 WP_000168388.1 ribbon-helix-helix domain-containing protein -
MPK88_RS20015 (4069948) 4069948..4070493 - 546 WP_223151225.1 helix-turn-helix domain-containing protein -
MPK88_RS20025 (4071250) 4071250..4072269 + 1020 WP_000152558.1 NAD(P)-dependent alcohol dehydrogenase -
MPK88_RS20030 (4072297) 4072297..4072827 - 531 WP_000896758.1 gluconokinase -
MPK88_RS20035 (4073044) 4073044..4074075 + 1032 WP_000453347.1 L-idonate 5-dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 4067346..4070448 3102


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(9-79)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11845.67 Da        Isoelectric Point: 6.7228

>T239415 WP_001199743.1 NZ_CP094269:c4069272-4068964 [Salmonella enterica subsp. enterica serovar Enteritidis]
MQYMVYRNKGNSKAYPYLLDVQSDIIDELHTRMVIPLFPVSRLVNNPVKRLTPTLNVEGNDYLVMTHEMASIRLSQIGDE
VMDVRSHRQTIKNALDFIFDGF

Download         Length: 309 bp

>T239415 NZ_LR890714:261346-261453 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8719.90 Da        Isoelectric Point: 6.4633

>AT239415 WP_001118105.1 NZ_CP094269:c4069514-4069275 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPGMSVRHKKTVSVTLEPALLQQARDAGINLSAVLTAALKEEISATGVERWKAENRAGLQELNRITDEHGLLSDDYRTF

Download         Length: 240 bp

>AT239415 NZ_LR890714:c261288-261234 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C6Z9V9

Antitoxin

Source ID Structure
AlphaFold DB A0A5H9SZK8

References