Detailed information of TA system TA238859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 729320..729958
• Replicon: chromosome
• Accession: NZ_CP093548
• Organism: Escherichia coli strain YL03

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: MPJ80_RS03715
• Protein ID: WP_001306887.1
• Coordinates: 729782..729958 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MPJ80_RS03710
• Protein ID: WP_001270286.1
• Coordinates: 729320..729736 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPJ80_RS03690 (724472)	724472..725413	-	942	WP_001251315.1	ABC transporter permease	-
MPJ80_RS03695 (725414)	725414..726427	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
MPJ80_RS03700 (726445)	726445..727590	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
MPJ80_RS03705 (727835)	727835..729241	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
MPJ80_RS03710 (729320)	729320..729736	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MPJ80_RS03715 (729782)	729782..729958	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MPJ80_RS03720 (730180)	730180..730410	+	231	WP_000494241.1	YncJ family protein	-
MPJ80_RS03725 (730502)	730502..732463	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MPJ80_RS03730 (732536)	732536..733072	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
MPJ80_RS03735 (733164)	733164..734336	+	1173	WP_001523225.1	BenE family transporter YdcO	-
MPJ80_RS03740 (734444)	734444..734902	-	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	734444..734902	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T238859 WP_001306887.1 NZ_CP093548:c729958-729782 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T238859 NZ_LR890508:3814190-3814486 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT238859 WP_001270286.1 NZ_CP093548:c729736-729320 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT238859 NZ_LR890508:3814488-3814883 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76