Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 35786..36429 Replicon plasmid pVA162-42
Accession NZ_CP093493
Organism Klebsiella pneumoniae strain VA126

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag MO410_RS28410 Protein ID WP_000754566.1
Coordinates 36013..36429 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag MO410_RS28405 Protein ID WP_001261276.1
Coordinates 35786..36016 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO410_RS28370 (MO410_28340) 30905..31174 + 270 WP_000339857.1 hypothetical protein -
MO410_RS28375 (MO410_28345) 31351..32217 - 867 WP_004118283.1 replication initiation protein -
MO410_RS28380 (MO410_28350) 32747..32851 + 105 WP_032409716.1 hypothetical protein -
MO410_RS28385 (MO410_28355) 32980..33237 + 258 WP_000764642.1 hypothetical protein -
MO410_RS28390 (MO410_28360) 33295..34071 - 777 WP_000015958.1 site-specific integrase -
MO410_RS28395 (MO410_28365) 34068..34811 - 744 WP_000129823.1 hypothetical protein -
MO410_RS28400 (MO410_28370) 34862..35212 - 351 WP_000493378.1 hypothetical protein -
MO410_RS28405 (MO410_28375) 35786..36016 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MO410_RS28410 (MO410_28380) 36013..36429 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
MO410_RS28415 (MO410_28385) 36633..37489 + 857 Protein_37 IS3-like element ISEc15 family transposase -
MO410_RS28420 (MO410_28390) 37771..37971 + 201 Protein_38 transposase -
MO410_RS28425 (MO410_28395) 37994..38416 + 423 WP_032446862.1 hypothetical protein -
MO410_RS28430 (MO410_28400) 38425..38922 + 498 WP_032446861.1 hypothetical protein -
MO410_RS28435 (MO410_28405) 39059..39394 - 336 Protein_41 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-2 / sul1 / qacE / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 - 1..41608 41608


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T238773 WP_000754566.1 NZ_CP093493:36013-36429 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T238773 NZ_LR890471:4099535-4099753 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT238773 WP_001261276.1 NZ_CP093493:35786-36016 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT238773 NZ_LR890471:4099134-4099508 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References