Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 199727..200466 Replicon chromosome
Accession NZ_CP093486
Organism Klebsiella pneumoniae strain VA172

Toxin (Protein)


Gene name tacT Uniprot ID A0A4U9WP91
Locus tag MO413_RS00980 Protein ID WP_004901283.1
Coordinates 199981..200466 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MO413_RS00975 Protein ID WP_003026799.1
Coordinates 199727..199993 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO413_RS00960 (195229) 195229..197298 + 2070 WP_002922127.1 glycine--tRNA ligase subunit beta -
MO413_RS00965 (197596) 197596..198965 + 1370 WP_087661561.1 IS3 family transposase -
MO413_RS00970 (199166) 199166..199594 + 429 WP_072001701.1 GFA family protein -
MO413_RS00975 (199727) 199727..199993 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MO413_RS00980 (199981) 199981..200466 + 486 WP_004901283.1 GNAT family N-acetyltransferase Toxin
MO413_RS00985 (200791) 200791..201837 - 1047 WP_004173931.1 IS481-like element ISKpn28 family transposase -
MO413_RS00990 (201911) 201911..202063 + 153 WP_012967075.1 type I toxin-antitoxin system toxin HokA -
MO413_RS00995 (202365) 202365..203984 + 1620 WP_040237813.1 ATP-binding cassette domain-containing protein -
MO413_RS01000 (204083) 204083..204295 - 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
MO413_RS01005 (204547) 204547..204837 - 291 WP_002921931.1 HTH-type transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 198333..201837 3504


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17594.32 Da        Isoelectric Point: 10.1788

>T238736 WP_004901283.1 NZ_CP093486:199981-200466 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLNQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Download         Length: 486 bp

>T238736 NZ_LR890466:366411-366514 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT238736 WP_003026799.1 NZ_CP093486:199727-199993 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT238736 NZ_LR890466:c366552-366281 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4U9WP91

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References