Detailed information of TA system TA238727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2785218..2786806
• Replicon: chromosome
• Accession: NZ_CP093481
• Organism: Klebsiella pneumoniae strain VA564

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: MO411_RS13770
• Protein ID: WP_032436337.1
• Coordinates: 2785218..2786540 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: MO411_RS13775
• Protein ID: WP_002904790.1
• Coordinates: 2786540..2786806 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MO411_RS13755 (2780275)	2780275..2782776	+	2502	WP_087836359.1	fimbria/pilus outer membrane usher protein	-
MO411_RS13760 (2782764)	2782764..2783768	+	1005	WP_032436338.1	fimbrial protein	-
MO411_RS13765 (2783909)	2783909..2785201	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
MO411_RS13770 (2785218)	2785218..2786540	-	1323	WP_032436337.1	type II toxin-antitoxin system HipA family toxin	Toxin
MO411_RS13775 (2786540)	2786540..2786806	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MO411_RS13780 (2787193)	2787193..2787519	-	327	WP_032436336.1	hydroxyisourate hydrolase	-
MO411_RS13785 (2787516)	2787516..2788016	-	501	WP_032436335.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
MO411_RS13790 (2787994)	2787994..2789373	-	1380	Protein_2703	nucleobase:cation symporter-2 family protein	-
MO411_RS13795 (2789719)	2789719..2790873	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
MO411_RS13800 (2790854)	2790854..2791777	-	924	WP_283557593.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49106.39 Da
• Isoelectric Point: 7.5617

>T238727 WP_032436337.1 NZ_CP093481:c2786540-2785218 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGDHWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T238727 NZ_LR890464:1995994-1996096 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT238727 WP_002904790.1 NZ_CP093481:c2786806-2786540 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT238727 NZ_LR890464:c1996103-1995958 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8