Detailed information of TA system TA238715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 142..616
• Replicon: plasmid pVA569-7
• Accession: NZ_CP093479
• Organism: Klebsiella pneumoniae strain VA569

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: S1DZ70
• Locus tag: MO417_RS28435
• Protein ID: WP_000262326.1
• Coordinates: 142..408 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: MO417_RS28440
• Protein ID: WP_000879771.1
• Coordinates: 392..616 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MO417_RS28435 (MO417_28410)	142..408	-	267	WP_000262326.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MO417_RS28440 (MO417_28415)	392..616	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
MO417_RS28445 (MO417_28420)	1464..1676	-	213	WP_000137423.1	MbeD family mobilization/exclusion protein	-
MO417_RS28450 (MO417_28425)	1680..2165	-	486	WP_013631087.1	MbeB family mobilization protein	-
MO417_RS28455 (MO417_28430)	2203..2634	-	432	Protein_5	relaxase/mobilization nuclease domain-containing protein	-
MO417_RS28460 (MO417_28435)	2843..3163	-	321	WP_045265547.1	MobC family plasmid mobilization relaxosome protein	-
MO417_RS28465 (MO417_28440)	3855..3989	+	135	WP_165451377.1	replication initiation protein	-
MO417_RS28470 (MO417_28445)	4195..4344	-	150	WP_080090625.1	colicin release lysis protein	-
MO417_RS28475 (MO417_28450)	4429..4686	-	258	WP_022644819.1	colicin E3-like toxin immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..6871	6871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10385.00 Da
• Isoelectric Point: 9.5211

>T238715 WP_000262326.1 NZ_CP093479:c408-142 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRILCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T238715 NZ_LR890457:c4769851-4769567 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT238715 WP_000879771.1 NZ_CP093479:c616-392 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT238715 NZ_LR890457:c4770083-4769841 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: S1DZ70

Antitoxin

• AlphaFold DB: S1EJF5