Detailed information of TA system TA023834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3432509..3433152
• Replicon: chromosome
• Accession: NC_012731
• Organism: Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A4GZE5
• Locus tag: KP1_RS16855
• Protein ID: WP_015874977.1
• Coordinates: 3432736..3433152 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A4GZE4
• Locus tag: KP1_RS16850
• Protein ID: WP_015874976.1
• Coordinates: 3432509..3432739 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KP1_RS16815	3428221..3428406	+	186	WP_000205185.1	AlpA family transcriptional regulator	-
KP1_RS27965	3428428..3428610	+	183	WP_072001695.1	hypothetical protein	-
KP1_RS16820	3428595..3429176	+	582	WP_000937857.1	DUF2857 domain-containing protein	-
KP1_RS27970	3429166..3429375	+	210	WP_032447696.1	TraR/DksA family transcriptional regulator	-
KP1_RS16830	3429618..3429731	+	114	WP_015874970.1	hypothetical protein	-
KP1_RS16835	3429775..3430501	-	727	Protein_3209	IS21-like element IS100kyp family helper ATPase IstB	-
KP1_RS16840	3430596..3431549	-	954	Protein_3210	IS21 family transposase	-
KP1_RS16845	3431686..3432066	-	381	WP_015874975.1	YrzE family protein	-
KP1_RS16850	3432509..3432739	+	231	WP_015874976.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KP1_RS16855	3432736..3433152	+	417	WP_015874977.1	type II toxin-antitoxin system VapC family toxin	Toxin
KP1_RS16860	3433188..3433433	+	246	WP_032447699.1	hypothetical protein	-
KP1_RS16865	3433588..3435762	-	2175	WP_015874979.1	siderophore salmochelin receptor IroN	-
KP1_RS16870	3436634..3437749	+	1116	WP_015874980.1	salmochelin biosynthesis C-glycosyltransferase IroB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA / iroN / iroB / iroC / iroD / rmpA	3395820..3472027	76207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14945.39 Da
• Isoelectric Point: 8.5464

>T23834 WP_015874977.1 NC_012731:3432736-3433152 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAGLRGDRIVVSAVTYAEMRFGATGPKASPHHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T23834 NC_012731:3432736-3433152 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGGGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCACCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTACCGGGCCTGGTGCTGG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8749.80 Da
• Isoelectric Point: 4.3533

>AT23834 WP_015874976.1 NC_012731:3432509-3432739 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMVEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT23834 NC_012731:3432509-3432739 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGTGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A4GZE5

Antitoxin

• AlphaFold DB: A4GZE4