Detailed information of TA system TA238260

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2931411..2932242
• Replicon: chromosome
• Accession: NZ_CP093368
• Organism: Escherichia coli strain EV36 mutant 1a

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: L0S95_RS14350
• Protein ID: WP_000854814.1
• Coordinates: 2931868..2932242 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: L0S95_RS14345
• Protein ID: WP_001285584.1
• Coordinates: 2931411..2931779 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0S95_RS14330 (2929078)	2929078..2930610	+	1533	WP_001350525.1	protein YeeR	-
L0S95_RS14335 (2930607)	2930607..2931053	+	447	WP_000187523.1	RadC family protein	-
L0S95_RS14340 (2931116)	2931116..2931337	+	222	WP_000692323.1	DUF987 domain-containing protein	-
L0S95_RS14345 (2931411)	2931411..2931779	+	369	WP_001285584.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
L0S95_RS14350 (2931868)	2931868..2932242	+	375	WP_000854814.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
L0S95_RS14355 (2932239)	2932239..2932433	+	195	WP_000988600.1	DUF5983 family protein	-
L0S95_RS14360 (2932479)	2932479..2932559	+	81	Protein_2819	hypothetical protein	-
L0S95_RS14365 (2932848)	2932848..2932976	-	129	Protein_2820	transposase domain-containing protein	-
L0S95_RS14370 (2933096)	2933096..2933230	+	135	WP_001297944.1	EutP/PduV family microcompartment system protein	-
L0S95_RS14375 (2933331)	2933331..2933660	-	330	WP_000450409.1	DUF496 family protein	-
L0S95_RS14380 (2933832)	2933832..2934890	-	1059	WP_001200891.1	FUSC family protein	-
L0S95_RS14385 (2935088)	2935088..2935561	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
L0S95_RS14390 (2935680)	2935680..2936846	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T238260 WP_000854814.1 NZ_CP093368:2931868-2932242 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T238260 NZ_LR890288:3720882-3721160 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT238260 WP_001285584.1 NZ_CP093368:2931411-2931779 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT238260 NZ_LR890288:3720619-3720879 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6