Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 42747..43372 Replicon chromosome
Accession NC_012731
Organism Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag KP1_RS00200 Protein ID WP_012737091.1
Coordinates 42989..43372 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KP1_RS00195 Protein ID WP_004150355.1
Coordinates 42747..42989 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP1_RS00170 38738..39337 + 600 WP_004151865.1 glucose-1-phosphatase -
KP1_RS00175 39331..40191 + 861 WP_004146232.1 virulence factor BrkB family protein -
KP1_RS00180 40188..40625 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
KP1_RS00185 40670..41611 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KP1_RS00190 41625..42542 - 918 WP_012737090.1 alpha/beta hydrolase -
KP1_RS00195 42747..42989 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
KP1_RS00200 42989..43372 + 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
KP1_RS00205 43546..44475 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KP1_RS00210 44472..45107 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KP1_RS00215 45104..46006 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T23821 WP_012737091.1 NC_012731:42989-43372 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T23821 NC_012731:42989-43372 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT23821 WP_004150355.1 NC_012731:42747-42989 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT23821 NC_012731:42747-42989 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References