Detailed information of TA system TA238091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1941..2488
• Replicon: plasmid pHUM3355_C
• Accession: NZ_CP093320
• Organism: Escherichia coli strain HUM3355

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: MLA23_RS24535
• Protein ID: WP_244442846.1
• Coordinates: 2210..2488 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: MLA23_RS24530
• Protein ID: WP_000079941.1
• Coordinates: 1941..2210 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLA23_RS24520 (1)	1..1032	+	1032	WP_000907873.1	plasmid replication initiator RepA	-
MLA23_RS24525 (1728)	1728..1886	+	159	WP_000841008.1	hypothetical protein	-
MLA23_RS24530 (1941)	1941..2210	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
MLA23_RS24535 (2210)	2210..2488	+	279	WP_244442846.1	type II toxin-antitoxin system toxin YacB	Toxin
MLA23_RS24540 (2528)	2528..3376	+	849	WP_001057991.1	3'-5' exonuclease	-
MLA23_RS24545 (3493)	3493..3966	+	474	WP_001334658.1	hypothetical protein	-
MLA23_RS24550 (4443)	4443..4709	-	267	WP_000955263.1	hypothetical protein	-
MLA23_RS24555 (4709)	4709..5203	-	495	WP_001459505.1	hypothetical protein	-
MLA23_RS24560 (5259)	5259..5861	-	603	WP_000517695.1	ProQ/FINO family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2	-	1..96053	96053

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10761.43 Da
• Isoelectric Point: 9.7617

>T238091 WP_244442846.1 NZ_CP093320:2210-2488 [Escherichia coli]
MEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T238091 NZ_LR890204:2372517-2372834 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT238091 WP_000079941.1 NZ_CP093320:1941-2210 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT238091 NZ_LR890204:2372910-2373206 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCCGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCAGCCAACCGGTGCAGCCAGAAAA
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5