Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3469297..3469915 Replicon chromosome
Accession NZ_CP093317
Organism Escherichia coli strain HUM3355

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MLA23_RS16780 Protein ID WP_001291435.1
Coordinates 3469697..3469915 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MLA23_RS16775 Protein ID WP_000344800.1
Coordinates 3469297..3469671 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MLA23_RS16765 (3464386) 3464386..3465579 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MLA23_RS16770 (3465602) 3465602..3468751 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
MLA23_RS16775 (3469297) 3469297..3469671 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MLA23_RS16780 (3469697) 3469697..3469915 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MLA23_RS16785 (3470087) 3470087..3470638 + 552 WP_000102564.1 maltose O-acetyltransferase -
MLA23_RS16790 (3470754) 3470754..3471224 + 471 WP_000136192.1 YlaC family protein -
MLA23_RS16795 (3471388) 3471388..3472938 + 1551 WP_263852469.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MLA23_RS16800 (3472980) 3472980..3473333 - 354 WP_000878140.1 DUF1428 domain-containing protein -
MLA23_RS16810 (3473712) 3473712..3474023 + 312 WP_000409911.1 MGMT family protein -
MLA23_RS16815 (3474054) 3474054..3474626 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T238084 WP_001291435.1 NZ_CP093317:3469697-3469915 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T238084 NZ_LR890202:4298464-4298811 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGAATACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT238084 WP_000344800.1 NZ_CP093317:3469297-3469671 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT238084 NZ_LR890202:4298811-4299110 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References