Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2359292..2359930 Replicon chromosome
Accession NZ_CP093317
Organism Escherichia coli strain HUM3355

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag MLA23_RS11220 Protein ID WP_000813794.1
Coordinates 2359754..2359930 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag MLA23_RS11215 Protein ID WP_001270286.1
Coordinates 2359292..2359708 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MLA23_RS11195 (2354444) 2354444..2355385 - 942 WP_001251313.1 ABC transporter permease -
MLA23_RS11200 (2355386) 2355386..2356399 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
MLA23_RS11205 (2356417) 2356417..2357562 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
MLA23_RS11210 (2357807) 2357807..2359213 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
MLA23_RS11215 (2359292) 2359292..2359708 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
MLA23_RS11220 (2359754) 2359754..2359930 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
MLA23_RS11225 (2360152) 2360152..2360382 + 231 WP_000494244.1 YncJ family protein -
MLA23_RS11230 (2360474) 2360474..2362435 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
MLA23_RS11235 (2362508) 2362508..2363044 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
MLA23_RS11240 (2363136) 2363136..2364311 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T238083 WP_000813794.1 NZ_CP093317:c2359930-2359754 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T238083 NZ_LR890202:4272495-4272863 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT238083 WP_001270286.1 NZ_CP093317:c2359708-2359292 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT238083 NZ_LR890202:4272277-4272498 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References