Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 863622..864420 | Replicon | chromosome |
| Accession | NZ_CP093283 | ||
| Organism | Escherichia coli strain EC-14-2-9 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | U9XMP3 |
| Locus tag | MNY25_RS04240 | Protein ID | WP_000854735.1 |
| Coordinates | 863622..863999 (-) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A1M1EW42 |
| Locus tag | MNY25_RS04245 | Protein ID | WP_032153712.1 |
| Coordinates | 864046..864420 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MNY25_RS04210 (859587) | 859587..860852 | - | 1266 | WP_001218773.1 | integrase arm-type DNA-binding domain-containing protein | - |
| MNY25_RS04215 (861316) | 861316..861642 | - | 327 | WP_000779483.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| MNY25_RS04220 (861639) | 861639..861902 | - | 264 | WP_025492088.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| MNY25_RS04225 (861974) | 861974..862840 | - | 867 | WP_032153714.1 | DUF4942 domain-containing protein | - |
| MNY25_RS04230 (862925) | 862925..863122 | - | 198 | WP_000839265.1 | DUF957 domain-containing protein | - |
| MNY25_RS04235 (863134) | 863134..863625 | - | 492 | WP_023155722.1 | DUF5983 family protein | - |
| MNY25_RS04240 (863622) | 863622..863999 | - | 378 | WP_000854735.1 | TA system toxin CbtA family protein | Toxin |
| MNY25_RS04245 (864046) | 864046..864420 | - | 375 | WP_032153712.1 | type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| MNY25_RS04250 (864500) | 864500..864721 | - | 222 | WP_000692345.1 | DUF987 domain-containing protein | - |
| MNY25_RS04255 (864790) | 864790..865266 | - | 477 | WP_001186715.1 | RadC family protein | - |
| MNY25_RS04260 (865282) | 865282..865767 | - | 486 | WP_032153711.1 | antirestriction protein | - |
| MNY25_RS04265 (865859) | 865859..866677 | - | 819 | WP_001234693.1 | DUF932 domain-containing protein | - |
| MNY25_RS04270 (866768) | 866768..866977 | - | 210 | WP_023155727.1 | DUF905 family protein | - |
| MNY25_RS04275 (867007) | 867007..867684 | - | 678 | WP_023155728.1 | hypothetical protein | - |
| MNY25_RS04280 (867803) | 867803..868687 | - | 885 | WP_024166630.1 | 50S ribosome-binding GTPase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR | rfaE / gspC / gspD / gspE / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / papI | 687767..893129 | 205362 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14061.07 Da Isoelectric Point: 8.2904
>T237997 WP_000854735.1 NZ_CP093283:c863999-863622 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ
Download Length: 378 bp
>T237997 NZ_LR883966:c40344-40057 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13697.51 Da Isoelectric Point: 6.6240
>AT237997 WP_032153712.1 NZ_CP093283:c864420-864046 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYMAVYPTLAPATTS
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYMAVYPTLAPATTS
Download Length: 375 bp
>AT237997 NZ_LR883966:c40592-40341 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U9XMP3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1M1EW42 |