Detailed information of TA system TA237968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 39007..39743
• Replicon: plasmid unnamed1
• Accession: NZ_CP093272
• Organism: Klebsiella oxytoca strain C-21-18

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: MN553_RS28015
• Protein ID: WP_004098919.1
• Coordinates: 39007..39489 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A2J5Q9C0
• Locus tag: MN553_RS28020
• Protein ID: WP_004098921.1
• Coordinates: 39477..39743 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN553_RS27990 (MN553_27990)	34397..34702	+	306	WP_012540235.1	PTS sugar transporter subunit IIB	-
MN553_RS27995 (MN553_27995)	34722..36056	+	1335	WP_049594012.1	PTS sugar transporter subunit IIC	-
MN553_RS28000 (MN553_28000)	36095..37474	+	1380	WP_049594011.1	glycoside hydrolase family 1 protein	-
MN553_RS28005 (MN553_28005)	37485..37796	+	312	WP_049594032.1	PTS lactose/cellobiose transporter subunit IIA	-
MN553_RS28010 (MN553_28010)	38177..38848	+	672	WP_224239842.1	hypothetical protein	-
MN553_RS28015 (MN553_28015)	39007..39489	-	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
MN553_RS28020 (MN553_28020)	39477..39743	-	267	WP_004098921.1	DUF1778 domain-containing protein	Antitoxin
MN553_RS28025 (MN553_28025)	39919..40173	-	255	WP_004098924.1	hypothetical protein	-
MN553_RS28030 (MN553_28030)	40249..40506	-	258	WP_004098928.1	hypothetical protein	-
MN553_RS28035 (MN553_28035)	40555..40758	-	204	WP_004098931.1	HHA domain-containing protein	-
MN553_RS28040 (MN553_28040)	41096..41530	-	435	WP_004098933.1	hypothetical protein	-
MN553_RS28045 (MN553_28045)	41746..43146	-	1401	WP_004098935.1	copper resistance membrane spanning protein PcoS	-
MN553_RS28050 (MN553_28050)	43143..43823	-	681	WP_004098938.1	copper response regulator transcription factor PcoR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..200578	200578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T237968 WP_004098919.1 NZ_CP093272:c39489-39007 [Klebsiella oxytoca]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T237968 NZ_LR883050:c4119747-4119460 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10105.49 Da
• Isoelectric Point: 4.5141

>AT237968 WP_004098921.1 NZ_CP093272:c39743-39477 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT237968 NZ_LR883050:c4119473-4119171 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928

Antitoxin

• AlphaFold DB: A0A2J5Q9C0