Detailed information of TA system TA237949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 267224..267932
• Replicon: plasmid pW1-a
• Accession: NZ_CP093256
• Organism: Moellerella wisconsensis strain W1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MNY70_RS17665
• Protein ID: WP_241502024.1
• Coordinates: 267543..267932 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4D7IVZ1
• Locus tag: MNY70_RS17660
• Protein ID: WP_067421833.1
• Coordinates: 267224..267553 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY70_RS17640 (MNY70_17640)	263395..263946	+	552	WP_137022556.1	hypothetical protein	-
MNY70_RS17645 (MNY70_17645)	263996..265135	-	1140	Protein_328	ISAs1 family transposase	-
MNY70_RS17650 (MNY70_17650)	265367..267016	+	1650	WP_241502023.1	ATP-binding protein	-
MNY70_RS17655 (MNY70_17655)	267003..267185	+	183	WP_137022558.1	hypothetical protein	-
MNY70_RS17660 (MNY70_17660)	267224..267553	-	330	WP_067421833.1	helix-turn-helix domain-containing protein	Antitoxin
MNY70_RS17665 (MNY70_17665)	267543..267932	-	390	WP_241502024.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MNY70_RS17670 (MNY70_17670)	268075..268683	+	609	WP_137022560.1	hypothetical protein	-
MNY70_RS17675 (MNY70_17675)	268737..269957	+	1221	WP_137022561.1	hypothetical protein	-
MNY70_RS17680 (MNY70_17680)	270050..270496	+	447	WP_137022562.1	hypothetical protein	-
MNY70_RS17685 (MNY70_17685)	270541..270681	+	141	WP_175403207.1	hypothetical protein	-
MNY70_RS17690 (MNY70_17690)	270716..271030	+	315	WP_241502025.1	hypothetical protein	-
MNY70_RS17695 (MNY70_17695)	271034..271822	+	789	WP_137022564.1	M48 family metallopeptidase	-
MNY70_RS17700 (MNY70_17700)	271890..272567	+	678	WP_137022565.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / blaCTX-M-1 / floR / aph(6)-Id / aph(3'')-Ib / aadA14 / lnu(G) / sul2	-	1..331804	331804

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-93)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14451.50 Da
• Isoelectric Point: 6.4817

>T237949 WP_241502024.1 NZ_CP093256:c267932-267543 [Moellerella wisconsensis]
VAFYKISSFVRSTKKIPITDKQLLDAAHEVQQGQFEADLGGGVIKKRLAMKGQGKSGSVRVIIFFKINNHLFFADGWTKN
TVNAKGTKEIEDDELETYKKLAAEFLNFDGEIINNLISKGILEEITDEQ

Nucleotide

• Length: 390 bp

>T237949 NZ_LR883012:3850475-3850753 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12062.15 Da
• Isoelectric Point: 10.2039

>AT237949 WP_067421833.1 NZ_CP093256:c267553-267224 [Moellerella wisconsensis]
MNNKLNNMLELAKELNAAGASPESLVKDIESTIRAREINDKIKEVSAMSGSEIKMLRTKYCMSQSVLARALGMSKESISK
WERGEKKPSGPALRMLRILESKGPEVLVV

Nucleotide

• Length: 330 bp

>AT237949 NZ_LR883012:3850212-3850472 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4D7IVZ1