Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 130764..131415 Replicon plasmid pW1-a
Accession NZ_CP093256
Organism Moellerella wisconsensis strain W1

Toxin (Protein)


Gene name higB Uniprot ID A0A1B8HBJ1
Locus tag MNY70_RS16740 Protein ID WP_067423956.1
Coordinates 130764..131114 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1B8HBM2
Locus tag MNY70_RS16745 Protein ID WP_067423953.1
Coordinates 131116..131415 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY70_RS16720 (MNY70_16720) 127359..127958 - 600 WP_213914587.1 DUF1788 domain-containing protein -
MNY70_RS16725 (MNY70_16725) 127955..128554 - 600 WP_241501906.1 DUF1819 family protein -
MNY70_RS16730 (MNY70_16730) 128564..129466 - 903 WP_241502041.1 WYL domain-containing protein -
MNY70_RS16735 (MNY70_16735) 130008..130568 - 561 WP_213914584.1 recombinase family protein -
MNY70_RS16740 (MNY70_16740) 130764..131114 + 351 WP_067423956.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MNY70_RS16745 (MNY70_16745) 131116..131415 + 300 WP_067423953.1 helix-turn-helix domain-containing protein Antitoxin
MNY70_RS16750 (MNY70_16750) 131444..132280 + 837 WP_109910744.1 hypothetical protein -
MNY70_RS16755 (MNY70_16755) 132315..132716 - 402 WP_067423949.1 hypothetical protein -
MNY70_RS16760 (MNY70_16760) 132817..133194 + 378 WP_109910745.1 hypothetical protein -
MNY70_RS16765 (MNY70_16765) 133148..133672 - 525 WP_067423945.1 hypothetical protein -
MNY70_RS16770 (MNY70_16770) 133706..133924 - 219 WP_109910747.1 hypothetical protein -
MNY70_RS16775 (MNY70_16775) 133981..134217 - 237 WP_067423943.1 hypothetical protein -
MNY70_RS16780 (MNY70_16780) 134198..134512 - 315 WP_082981384.1 hypothetical protein -
MNY70_RS16785 (MNY70_16785) 134589..134798 - 210 WP_109910748.1 hypothetical protein -
MNY70_RS16790 (MNY70_16790) 135015..135563 + 549 WP_241501905.1 PAAR domain-containing protein -
MNY70_RS16795 (MNY70_16795) 135578..135886 + 309 WP_241545130.1 PAAR domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / blaCTX-M-1 / floR / aph(6)-Id / aph(3'')-Ib / aadA14 / lnu(G) / sul2 - 1..331804 331804
- flank IS/Tn - - 130008..130568 560


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-84)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13453.50 Da        Isoelectric Point: 5.2661

>T237948 WP_067423956.1 NZ_CP093256:130764-131114 [Moellerella wisconsensis]
MWEILTRDLFDSWFEEQNEETQIEVLAVLMILREDGPNLGRPQVDTLKGSQFPNMKELRIQVGGHPIRACFAFDPIRRGI
VLCAGDKKGKDETRFYKKLIKMADAEYSAHLLEQEK

Download         Length: 351 bp

>T237948 NZ_LR883012:c3844413-3844015 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10888.61 Da        Isoelectric Point: 8.5841

>AT237948 WP_067423953.1 NZ_CP093256:131116-131415 [Moellerella wisconsensis]
MSTLNELLAKQSPEALAKIEARAEEIRREITLAKIREELNLSQSELAKSLGVSQPSIAKLENVDNDPKLSTLKRYIKALG
GELSIDVTLPNGKRIGLHL

Download         Length: 300 bp

>AT237948 NZ_LR883012:c3844709-3844416 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B8HBJ1

Antitoxin

Source ID Structure
AlphaFold DB A0A1B8HBM2

References