Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PRK09907-MazE |
| Location | 72275..72852 | Replicon | plasmid pW1-a |
| Accession | NZ_CP093256 | ||
| Organism | Moellerella wisconsensis strain W1 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | U5N651 |
| Locus tag | MNY70_RS16395 | Protein ID | WP_023159957.1 |
| Coordinates | 72520..72852 (+) | Length | 111 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | - |
| Locus tag | MNY70_RS16390 | Protein ID | WP_096864992.1 |
| Coordinates | 72275..72520 (+) | Length | 82 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MNY70_RS16360 (MNY70_16360) | 68817..69182 | - | 366 | WP_096865004.1 | arsenite efflux transporter metallochaperone ArsD | - |
| MNY70_RS16365 (MNY70_16365) | 69231..69566 | - | 336 | WP_096865005.1 | metalloregulator ArsR/SmtB family transcription factor | - |
| MNY70_RS16370 (MNY70_16370) | 69910..70278 | + | 369 | WP_135017200.1 | hypothetical protein | - |
| MNY70_RS16375 (MNY70_16375) | 70376..70603 | - | 228 | WP_135017201.1 | plasmid partition protein ParG | - |
| MNY70_RS16380 (MNY70_16380) | 70696..71319 | - | 624 | WP_135017202.1 | AAA family ATPase | - |
| MNY70_RS16385 (MNY70_16385) | 71592..72077 | - | 486 | WP_135017203.1 | hypothetical protein | - |
| MNY70_RS16390 (MNY70_16390) | 72275..72520 | + | 246 | WP_096864992.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| MNY70_RS16395 (MNY70_16395) | 72520..72852 | + | 333 | WP_023159957.1 | endoribonuclease MazF | Toxin |
| MNY70_RS16400 (MNY70_16400) | 72883..73263 | + | 381 | WP_096864991.1 | hypothetical protein | - |
| MNY70_RS16405 (MNY70_16405) | 73350..73493 | - | 144 | WP_071547955.1 | Hok/Gef family protein | - |
| MNY70_RS16410 (MNY70_16410) | 73813..74271 | - | 459 | WP_241545126.1 | hypothetical protein | - |
| MNY70_RS16415 (MNY70_16415) | 74367..74621 | - | 255 | WP_135017207.1 | hypothetical protein | - |
| MNY70_RS16420 (MNY70_16420) | 74660..74929 | - | 270 | WP_241545127.1 | hypothetical protein | - |
| MNY70_RS16425 (MNY70_16425) | 75224..76177 | + | 954 | WP_166268230.1 | site-specific integrase | - |
| MNY70_RS16430 (MNY70_16430) | 76193..77011 | + | 819 | WP_241543873.1 | DUF4365 domain-containing protein | - |
| MNY70_RS16435 (MNY70_16435) | 77032..77598 | + | 567 | WP_241501928.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | mph(A) / blaCTX-M-1 / floR / aph(6)-Id / aph(3'')-Ib / aadA14 / lnu(G) / sul2 | - | 1..331804 | 331804 | |
| - | flank | IS/Tn | - | - | 77688..78635 | 947 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 111 a.a. Molecular weight: 12097.88 Da Isoelectric Point: 7.0813
>T237947 WP_023159957.1 NZ_CP093256:72520-72852 [Moellerella wisconsensis]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG
Download Length: 333 bp
>T237947 NZ_LR883012:3562604-3562822 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
Antitoxin
Download Length: 82 a.a. Molecular weight: 8800.23 Da Isoelectric Point: 5.0508
>AT237947 WP_096864992.1 NZ_CP093256:72275-72520 [Moellerella wisconsensis]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLENLLAGITPENIHEKVDFGARTGKEL
I
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLENLLAGITPENIHEKVDFGARTGKEL
I
Download Length: 246 bp
>AT237947 NZ_LR883012:3562204-3562578 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|