Detailed information of TA system TA237887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1988..2535
• Replicon: plasmid pEC-16-35-4
• Accession: NZ_CP093229
• Organism: Escherichia coli strain EC-16-35

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: MNY24_RS24925
• Protein ID: WP_013009346.1
• Coordinates: 2257..2535 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E0WH52
• Locus tag: MNY24_RS24920
• Protein ID: WP_000079932.1
• Coordinates: 1988..2257 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY24_RS24910	46..1077	+	1032	WP_000907881.1	plasmid replication initiator RepA	-
MNY24_RS24915	1775..1933	+	159	WP_000829588.1	hypothetical protein	-
MNY24_RS24920	1988..2257	+	270	WP_000079932.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
MNY24_RS24925	2257..2535	+	279	WP_013009346.1	type II toxin-antitoxin system toxin YacB	Toxin
MNY24_RS24930	2731..3027	-	297	WP_001419651.1	hypothetical protein	-
MNY24_RS24935	3372..3959	+	588	WP_001418189.1	serine acetyltransferase	-
MNY24_RS24940	4241..4834	-	594	WP_001401779.1	ProQ/FINO family protein	-
MNY24_RS24945	5188..6534	+	1347	WP_001132021.1	protein YagA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..90339	90339
	-	flank	IS/Tn	-	-	6866..7570	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10748.35 Da
• Isoelectric Point: 8.4615

>T237887 WP_013009346.1 NZ_CP093229:2257-2535 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
VLRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T237887 NZ_LR882997:c3165930-3165547 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10268.58 Da
• Isoelectric Point: 7.2055

>AT237887 WP_000079932.1 NZ_CP093229:1988-2257 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVSDK

Nucleotide

• Length: 270 bp

>AT237887 NZ_LR882997:c3166391-3166011 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Antitoxin

• AlphaFold DB: A0A5U8RN22