Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3722148..3722689 Replicon chromosome
Accession NZ_CP093226
Organism Escherichia coli strain EC-16-35

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag MNY24_RS17965 Protein ID WP_000615983.1
Coordinates 3722411..3722689 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag MNY24_RS17960 Protein ID WP_000729703.1
Coordinates 3722148..3722408 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY24_RS17940 (3717823) 3717823..3718608 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
MNY24_RS17945 (3718580) 3718580..3720292 + 1713 Protein_3533 flagellar biosynthesis protein FlhA -
MNY24_RS17950 (3720516) 3720516..3721013 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
MNY24_RS17955 (3721189) 3721189..3721938 - 750 WP_000056849.1 C40 family peptidase -
MNY24_RS17960 (3722148) 3722148..3722408 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
MNY24_RS17965 (3722411) 3722411..3722689 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
MNY24_RS17970 (3722845) 3722845..3723585 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
MNY24_RS17975 (3723556) 3723556..3724323 - 768 WP_000333380.1 class II glutamine amidotransferase -
MNY24_RS17980 (3724529) 3724529..3725107 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T237870 WP_000615983.1 NZ_CP093226:3722411-3722689 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T237870 NZ_LR882990:3633582-3633800 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT237870 WP_000729703.1 NZ_CP093226:3722148-3722408 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT237870 NZ_LR882990:3633182-3633556 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGATCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAGGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References