Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 930067..930721 Replicon chromosome
Accession NZ_CP092973
Organism Escherichia coli strain WCHEC005215

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag MM176_RS04560 Protein ID WP_000244777.1
Coordinates 930314..930721 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MM176_RS04555 Protein ID WP_000354046.1
Coordinates 930067..930333 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MM176_RS04530 (925236) 925236..925979 + 744 WP_000951964.1 SDR family oxidoreductase -
MM176_RS04535 (926036) 926036..927469 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
MM176_RS04540 (927514) 927514..927825 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
MM176_RS04545 (927989) 927989..928648 + 660 WP_000250274.1 hemolysin III family protein -
MM176_RS04550 (928844) 928844..929824 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MM176_RS04555 (930067) 930067..930333 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MM176_RS04560 (930314) 930314..930721 + 408 WP_000244777.1 protein YgfX Toxin
MM176_RS04565 (930761) 930761..931282 - 522 WP_001055874.1 flavodoxin FldB -
MM176_RS04570 (931394) 931394..932290 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MM176_RS04575 (932315) 932315..933025 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MM176_RS04580 (933031) 933031..934764 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T237440 WP_000244777.1 NZ_CP092973:930314-930721 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T237440 NZ_LR882057:c2576680-2576504 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT237440 WP_000354046.1 NZ_CP092973:930067-930333 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT237440 NZ_LR882057:c2576458-2576042 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGTGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References