Detailed information of TA system TA023731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 410582..411110
• Replicon: chromosome II
• Accession: NC_012583
• Organism: Vibrio cholerae O395

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: VC395_RS16315
• Protein ID: WP_000221352.1
• Coordinates: 410582..410872 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H9L4T4
• Locus tag: VC395_RS16320
• Protein ID: WP_000213183.1
• Coordinates: 410862..411110 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VC395_RS21045	406140..406265	+	126	WP_001944767.1	DUF645 family protein	-
VC395_RS21050	406281..406319	+	39	WP_106019119.1	hypothetical protein	-
VC395_RS16290	406532..407536	+	1005	WP_000964934.1	LD-carboxypeptidase	-
VC395_RS16295	407688..408134	+	447	WP_000128977.1	hypothetical protein	-
VC395_RS16300	408254..409183	+	930	WP_000335802.1	hypothetical protein	-
VC395_RS20640	409180..409290	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
VC395_RS16305	409318..409794	+	477	WP_001047171.1	GNAT family N-acetyltransferase	-
VC395_RS16310	409931..410446	+	516	WP_001201531.1	lipocalin family protein	-
VC395_RS16315	410582..410872	-	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VC395_RS16320	410862..411110	-	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
VC395_RS16335	411681..412334	+	654	WP_000036957.1	site-specific DNA-methyltransferase	-
VC395_RS16340	412605..413030	+	426	WP_000415748.1	hypothetical protein	-
VC395_RS16345	413238..413633	+	396	WP_000649527.1	DUF3465 domain-containing protein	-
VC395_RS16350	413784..414314	+	531	WP_000415562.1	hypothetical protein	-
VC395_RS16355	414452..414952	+	501	WP_000789723.1	hypothetical protein	-
VC395_RS16360	415108..415506	+	399	WP_001882293.1	hypothetical protein	-
VC395_RS16365	415606..416070	-	465	WP_000140244.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	344619..430484	85865

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T23731 WP_000221352.1 NC_012583:c410872-410582 [Vibrio cholerae O395]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T23731 NC_012583:c410872-410582 [Vibrio cholerae O395]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8937.28 Da
• Isoelectric Point: 4.1079

>AT23731 WP_000213183.1 NC_012583:c411110-410862 [Vibrio cholerae O395]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT23731 NC_012583:c411110-410862 [Vibrio cholerae O395]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A366A3W8