Detailed information of TA system TA023730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-Phd
• Location: 395426..395973
• Replicon: chromosome II
• Accession: NC_012583
• Organism: Vibrio cholerae O395

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0H3AD26
• Locus tag: VC395_RS16215
• Protein ID: WP_000807826.1
• Coordinates: 395671..395973 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9KM93
• Locus tag: VC395_RS16210
• Protein ID: WP_000861987.1
• Coordinates: 395426..395683 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VC395_RS16165	390909..391358	+	450	WP_000480555.1	hypothetical protein	-
VC395_RS16175	391653..391835	+	183	WP_001889223.1	DUF645 family protein	-
VC395_RS16180	392043..392735	+	693	WP_000780703.1	hypothetical protein	-
VC395_RS16185	392735..393295	+	561	Protein_382	hypothetical protein	-
VC395_RS16190	393410..393820	+	411	WP_000452031.1	hypothetical protein	-
VC395_RS16195	393915..394340	+	426	WP_000403003.1	GNAT family N-acetyltransferase	-
VC395_RS16200	394584..394820	+	237	WP_001065132.1	hypothetical protein	-
VC395_RS16210	395426..395683	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
VC395_RS16215	395671..395973	+	303	WP_000807826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VC395_RS16220	396135..396716	+	582	WP_001025027.1	hypothetical protein	-
VC395_RS20265	396707..396823	+	117	WP_114732087.1	DUF3265 domain-containing protein	-
VC395_RS20885	396813..396965	+	153	Protein_390	DUF3709 domain-containing protein	-
VC395_RS16225	396986..397207	+	222	WP_032074688.1	DUF3709 domain-containing protein	-
VC395_RS16230	397547..397909	+	363	WP_002034853.1	hypothetical protein	-
VC395_RS16235	398058..398984	+	927	WP_000377815.1	hypothetical protein	-
VC395_RS16240	399124..399510	+	387	WP_001015083.1	MmcQ/YjbR family DNA-binding protein	-
VC395_RS20270	399694..400017	+	324	WP_001999495.1	DUF3709 domain-containing protein	-
VC395_RS16245	400303..400698	+	396	WP_000870868.1	DUF3465 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	344619..430484	85865

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11725.65 Da
• Isoelectric Point: 5.1972

>T23730 WP_000807826.1 NC_012583:395671-395973 [Vibrio cholerae O395]
MAEIIWTEPALSDLNDIAEYIALENVVAAKQLVQTVFTKVERLADFPESGRVPLELEHLNYREVVVNPCRVFYKYDDAKV
RILFVMRAERDLRRLMLTKQ

Nucleotide

• Length: 303 bp

>T23730 NC_012583:395671-395973 [Vibrio cholerae O395]
ATGGCTGAAATAATTTGGACGGAGCCGGCTCTATCCGACCTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTCGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTT
TAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAATCCGTGTCGTGTTTTCTATAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9647.15 Da
• Isoelectric Point: 7.3178

>AT23730 WP_000861987.1 NC_012583:395426-395683 [Vibrio cholerae O395]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLIDVDDYEFMQNRLAILEGIARGERALADGKVVSHQDAKDRM
SKWLK

Nucleotide

• Length: 258 bp

>AT23730 NC_012583:395426-395683 [Vibrio cholerae O395]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGTGGAGAGCGAGCTCTAGCGGATGGCAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGCTGAAATAA

Structures

Toxin

• AlphaFold DB: A0A0H3AD26

Antitoxin

• AlphaFold DB: A0A1T4SLM9