Detailed information of TA system TA023724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 892961..894786
• Replicon: chromosome
• Accession: NC_012582
• Organism: Vibrio cholerae O395

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: VC395_RS04270
• Protein ID: WP_000392003.1
• Coordinates: 893425..894786 (-)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: VC395_RS04265
• Protein ID: WP_000804849.1
• Coordinates: 892961..893428 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VC395_RS04250	888241..888945	-	705	WP_001880605.1	NERD domain-containing protein	-
VC395_RS04255	889015..891951	-	2937	WP_001283873.1	DEAD/DEAH box helicase family protein	-
VC395_RS04260	891955..892533	-	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
VC395_RS04265	892961..893428	-	468	WP_000804849.1	helix-turn-helix domain-containing protein	Antitoxin
VC395_RS04270	893425..894786	-	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
VC395_RS04280	895390..896367	+	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
VC395_RS19750	896393..897073	-	681	Protein_767	IS5 family transposase	-
VC395_RS04295	897415..898935	-	1521	WP_000640055.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T23724 WP_000392003.1 NC_012582:c894786-893425 [Vibrio cholerae O395]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T23724 NC_012582:c894786-893425 [Vibrio cholerae O395]
ATGGAAGAGCTAACCGTACAAGCTTTTATCAGAGGTGAATGGATAGATATTGGTATCATCTCATTCCCTAAAAGTAGCCA
ACATAATTTTCGAGTGACTGAACTCAACTACCTCAGCGACTATGCGCTAGAACATTACGATAAAGATGACTTTCACGCTG
TTTCACTAAACCACCCTGTCTCATTCATTTTTGATGACATGGGTAAGCCTGGATGGTTAAAGTTTCTAGACGATATTATG
CCTAGTGGCGCTAGTAGACGATACTGGGTCAAACACCTAGATATTGAAGATCTTAGTTCTGATGAACAAGACTATGTTTT
GCTCAAATTTGGCACGATGTCGCCTATTGGTAACTTAAGAATAAAAGACTCCCTTCCTGAACGCTACGAAGTCGCAGACA
ACCTCTACTTCTCTGTTGATGATGTTAAAAATCGAGCAGGTGATTTCCTCGACTATGCACAGCAAAGAGGGGCAGCCGCA
GGTGGTGCAACAGGGGCTGGAGGTGAAGCGCCAAAACTGATCCTCCGATGTGGTTTCGATCATGGATCTGGCAGCGAAAA
AATATGGATTGATCCATACCAAGATGACAATAGCAGCCACGACTTACATTACTTAGTAAAGTACCCTAGAGGCTCAAGAA
GCACCATCGACTGTAATATTCTAAGAGCTGAGTTCTACTTCTACCACGAGCTAACTGAAATGGGAGTTGAAACGATCTCC
ACCGACGGTATGCGCTTGGAGGAAGGATTAAACTACCCTTCCTTATGGCTTCCTCGATTTGATGTTCAAATTATTGATCA
ACAAATAGAGCGATTTGGCATGGAATCAGTGTATTCCATTTTGAACAAAGGCGCTGGAGTCACTCTTGATCACGAAACAA
CAATCCGAACTCTCATAGAAAAAATTACTGAAAGTAATATGGTTAAACACCAAGGGTATAGGTTTGATACCCAAGCTTTC
GTTATTGAATGGGTGAAGAGAGATCTACTCAACATCTTGTTTGGTAATAGTGATAACCATGGCAGAAATACTTCATTCTT
AAAGGGTGATGGAGTCATAAAGCTTGCTCCCATTTACGATTTCGCACCAATGAAAGCCGATCCTGCAGGAATTCCAAGAA
CAACAAAATGGAAAGCCCCACTAGAGGTTGGCGGAACTTACGACTTTGTCGGCATTGCAGACACCTTATCTGATCTGGTG
CCAAAGGAAATGTTATTAAAAGAGCTAGCAATTACTGCAAGTAAATGCGTAGGCCTAAAGCAGAGACTCGCTTTACGAGG
AGTACCAGAACAAATCTTGGAGATGCCGGCCGTTGGACTTAATTATTTATCAGAAAAGCTCACTAAATGGGGGCTACTAT
GA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT23724 WP_000804849.1 NC_012582:c893428-892961 [Vibrio cholerae O395]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT23724 NC_012582:c893428-892961 [Vibrio cholerae O395]
ATGAAGCCAAGCAATAAAGAGTCCGTACAAGATACGGTAGCTCGTCTAAGAGAAAACCGAAATCAACAGCAGTCCAAGGT
TGGGAAGAATGCAACTCATTCGAGCAAAGAGCAAACACCACTCGATACTCCAACGAGATCGAAAGCCTCAAAAAACGCCA
AAGCAGTAAGTGCAGCAGAGCGCAAAACCGAGGCAAACAAAATCATCAAACACTTATTGCTTGGAGAGCTAACACAGGGC
CAAGCGCTGAAAAGTCTACGCATTAATATACTGGGACTCAAACAGGATGTATTCGCAAGACTTGTCGATGTCTCAAGAAA
AACACTCTCAGACATAGAGAATGATCGGGGCAGCTACAATACAGAGATTCTCAACAAGGTATTCAAACCATTTAGCTTAA
AAATCGGGCTGCTGCCTTCTTCCCCCGATGCGTTAAAATCGCTCTTAATGGATGGTGAGGATGGGTAA

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8