Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4145742..4146361 Replicon chromosome
Accession NZ_CP092717
Organism Klebsiella pneumoniae strain HvKp-su1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MKX18_RS20535 Protein ID WP_002892050.1
Coordinates 4146143..4146361 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag MKX18_RS20530 Protein ID WP_002892066.1
Coordinates 4145742..4146116 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKX18_RS20520 (MKX18_20520) 4140894..4142087 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MKX18_RS20525 (MKX18_20525) 4142110..4145256 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
MKX18_RS20530 (MKX18_20530) 4145742..4146116 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
MKX18_RS20535 (MKX18_20535) 4146143..4146361 + 219 WP_002892050.1 HHA domain-containing protein Toxin
MKX18_RS20540 (MKX18_20540) 4146520..4147086 + 567 WP_002892030.1 maltose O-acetyltransferase -
MKX18_RS20545 (MKX18_20545) 4147058..4147198 - 141 WP_004147370.1 hypothetical protein -
MKX18_RS20550 (MKX18_20550) 4147219..4147689 + 471 WP_002892026.1 YlaC family protein -
MKX18_RS20555 (MKX18_20555) 4147664..4149115 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
MKX18_RS20560 (MKX18_20560) 4149216..4149914 + 699 WP_002892021.1 GNAT family protein -
MKX18_RS20565 (MKX18_20565) 4149911..4150051 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MKX18_RS20570 (MKX18_20570) 4150051..4150314 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T237035 WP_002892050.1 NZ_CP092717:4146143-4146361 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T237035 NZ_LR778147:c1144016-1143552 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCTTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCGATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT237035 WP_002892066.1 NZ_CP092717:4145742-4146116 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT237035 NZ_LR778147:c1144351-1144016 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGATGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References