Detailed information of TA system TA023685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1493504..1494085
• Replicon: chromosome
• Accession: NC_012559
• Organism: Laribacter hongkongensis HLHK9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C1D7U8
• Locus tag: LHK_RS07110
• Protein ID: WP_012697024.1
• Coordinates: 1493804..1494085 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: LHK_RS07105
• Protein ID: WP_012697023.1
• Coordinates: 1493504..1493794 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHK_RS07080	1488623..1489084	+	462	WP_012697017.1	hypothetical protein	-
LHK_RS16485	1489097..1489339	+	243	WP_012697018.1	hypothetical protein	-
LHK_RS07085	1489406..1490011	+	606	WP_012697019.1	hypothetical protein	-
LHK_RS15895	1490011..1490829	+	819	Protein_1425	hypothetical protein	-
LHK_RS15260	1491388..1492635	+	1248	Protein_1426	hypothetical protein	-
LHK_RS07095	1492736..1493080	+	345	WP_012697021.1	toxin	-
LHK_RS07100	1493077..1493388	+	312	WP_012697022.1	XRE family transcriptional regulator	-
LHK_RS07105	1493504..1493794	-	291	WP_012697023.1	HigA family addiction module antidote protein	Antitoxin
LHK_RS07110	1493804..1494085	-	282	WP_012697024.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LHK_RS07115	1494202..1494801	+	600	WP_012697025.1	hypothetical protein	-
LHK_RS15265	1494812..1496962	+	2151	WP_012697026.1	hypothetical protein	-
LHK_RS07125	1496962..1497459	+	498	WP_012697027.1	hypothetical protein	-
LHK_RS16490	1497365..1498495	-	1131	WP_143714455.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10458.92 Da
• Isoelectric Point: 7.4203

>T23685 WP_012697024.1 NC_012559:c1494085-1493804 [Laribacter hongkongensis HLHK9]
MITSFCHKGLESFFLTGSKAGIRPDHAGKLQKQLTALDSARQPDDMAVPSWKLHLLKGDLAGHYAVTVNANWRLTFRFLP
TGDVELVDYQDYH

Nucleotide

• Length: 282 bp

>T23685 NC_012559:c1494085-1493804 [Laribacter hongkongensis HLHK9]
ATGATTACTTCGTTCTGTCACAAGGGACTCGAGTCGTTCTTCCTGACGGGCAGCAAGGCGGGTATCCGGCCTGACCACGC
AGGAAAACTTCAAAAGCAGCTGACCGCACTCGACTCGGCCAGACAACCAGACGATATGGCGGTTCCAAGCTGGAAACTCC
ACCTACTAAAAGGGGATCTGGCCGGACACTACGCTGTTACCGTCAATGCTAACTGGCGACTGACCTTCAGGTTTTTGCCC
ACTGGCGATGTGGAGTTGGTTGACTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10521.04 Da
• Isoelectric Point: 8.8465

>AT23685 WP_012697023.1 NC_012559:c1493794-1493504 [Laribacter hongkongensis HLHK9]
MSRMHNPAHPGTVLADWLDGISVTDAARKLGITRTALSRILHGHAGISADMAIRLSEALGTTPELWLGMQSAYDLWQAGQ
RPRPHVERMFFASQAT

Nucleotide

• Length: 291 bp

>AT23685 NC_012559:c1493794-1493504 [Laribacter hongkongensis HLHK9]
ATGAGCCGCATGCACAACCCGGCACACCCTGGAACCGTGCTGGCTGACTGGCTGGATGGAATCAGCGTTACCGACGCAGC
CAGGAAACTTGGTATTACACGGACGGCTTTATCCCGCATTCTGCACGGCCATGCCGGTATCAGTGCAGACATGGCCATTC
GTCTCTCGGAGGCACTGGGTACAACGCCAGAACTATGGCTTGGCATGCAGTCAGCCTACGATTTGTGGCAGGCCGGTCAG
CGCCCGCGCCCACACGTCGAGCGCATGTTCTTCGCATCACAGGCAACCTGA

Structures

Toxin

• AlphaFold DB: C1D7U8