Detailed information of TA system TA236827

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1787999..1788813
• Replicon: chromosome
• Accession: NZ_CP092639
• Organism: Escherichia coli strain HS

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7I6H0K3
• Locus tag: MJ392_RS08505
• Protein ID: WP_000854760.1
• Coordinates: 1787999..1788376 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MJ392_RS08510
• Protein ID: WP_000005490.1
• Coordinates: 1788466..1788813 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ392_RS08470 (1783519)	1783519..1783992	+	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-
MJ392_RS08475 (1784190)	1784190..1785248	+	1059	WP_001200895.1	FUSC family protein	-
MJ392_RS08480 (1785420)	1785420..1785749	+	330	WP_000450409.1	DUF496 family protein	-
MJ392_RS08485 (1785850)	1785850..1786215	-	366	WP_001280454.1	EutP/PduV family microcompartment system protein	-
MJ392_RS08490 (1786486)	1786486..1786875	-	390	WP_001445118.1	IS110 family transposase	-
MJ392_RS08495 (1787673)	1787673..1787753	-	81	Protein_1669	hypothetical protein	-
MJ392_RS08500 (1787853)	1787853..1788002	-	150	Protein_1670	DUF5983 family protein	-
MJ392_RS08505 (1787999)	1787999..1788376	-	378	WP_000854760.1	TA system toxin CbtA family protein	Toxin
MJ392_RS08510 (1788466)	1788466..1788813	-	348	WP_000005490.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MJ392_RS08515 (1788770)	1788770..1790470	-	1701	Protein_1673	AIDA repeat-containing protein	-
MJ392_RS08520 (1790842)	1790842..1791714	-	873	WP_001069714.1	GTPase family protein	-
MJ392_RS08525 (1791799)	1791799..1792716	-	918	WP_000250228.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-110)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14165.19 Da
• Isoelectric Point: 7.3249

>T236827 WP_000854760.1 NZ_CP092639:c1788376-1787999 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T236827 NZ_LR740758:c2092479-2092225 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 12816.73 Da
• Isoelectric Point: 8.1256

>AT236827 WP_000005490.1 NZ_CP092639:c1788813-1788466 [Escherichia coli]
MSETVTSGWQPPDRVPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQ
HTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 348 bp

>AT236827 NZ_LR740758:c2092727-2092476 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7I6H0K3