Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3997519..3998168 Replicon chromosome
Accession NZ_CP092635
Organism Enterobacter cloacae strain EFN743

Toxin (Protein)


Gene name higB Uniprot ID A0A5E1A383
Locus tag MKZ88_RS19380 Protein ID WP_013097692.1
Coordinates 3997519..3997881 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7Z1F7X8
Locus tag MKZ88_RS19385 Protein ID WP_058651676.1
Coordinates 3997869..3998168 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ88_RS19360 (MKZ88_19360) 3992853..3993824 - 972 WP_023620419.1 LacI family DNA-binding transcriptional regulator -
MKZ88_RS19365 (MKZ88_19365) 3993900..3995042 - 1143 WP_058651679.1 Gfo/Idh/MocA family oxidoreductase -
MKZ88_RS19370 (MKZ88_19370) 3995035..3996060 - 1026 WP_058651678.1 sugar phosphate isomerase/epimerase family protein -
MKZ88_RS19375 (MKZ88_19375) 3996076..3997314 - 1239 WP_058651677.1 MFS transporter -
MKZ88_RS19380 (MKZ88_19380) 3997519..3997881 + 363 WP_013097692.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MKZ88_RS19385 (MKZ88_19385) 3997869..3998168 + 300 WP_058651676.1 helix-turn-helix transcriptional regulator Antitoxin
MKZ88_RS19390 (MKZ88_19390) 3998292..3999680 - 1389 WP_058651675.1 phenylalanine transporter -
MKZ88_RS19395 (MKZ88_19395) 3999783..4001756 - 1974 WP_057071947.1 PhoX family phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13994.00 Da        Isoelectric Point: 7.3235

>T236817 WP_013097692.1 NZ_CP092635:3997519-3997881 [Enterobacter cloacae]
MWNVETTDRFDEWFFEQTTALKEDVLAAMHILSEFGPQLGRPFVDTVKASAFGNMKELRIQHRGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYKEMIALADVEYHRHLANKEAIWQR

Download         Length: 363 bp

>T236817 NZ_LR740758:c15606-15454 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10889.65 Da        Isoelectric Point: 8.6152

>AT236817 WP_058651676.1 NZ_CP092635:3997869-3998168 [Enterobacter cloacae]
MATLKELMAQQSAESQERIAAKVEVMRRIVALNQLRDELNISQTELAVAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GELSIDVTLPNGKRIAFEI

Download         Length: 300 bp

>AT236817 NZ_LR740758:15655-15712 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A383

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z1F7X8

References