Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5437803..5438428 Replicon chromosome
Accession NZ_CP092632
Organism Klebsiella variicola subsp. variicola strain ML.9ba2

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag MKK01_RS26110 Protein ID WP_008807903.1
Coordinates 5437803..5438186 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MKK01_RS26115 Protein ID WP_004150355.1
Coordinates 5438186..5438428 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKK01_RS26095 (MKK01_26095) 5435169..5436071 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MKK01_RS26100 (MKK01_26100) 5436068..5436703 + 636 WP_240289602.1 formate dehydrogenase cytochrome b556 subunit -
MKK01_RS26105 (MKK01_26105) 5436700..5437629 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MKK01_RS26110 (MKK01_26110) 5437803..5438186 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
MKK01_RS26115 (MKK01_26115) 5438186..5438428 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MKK01_RS26120 (MKK01_26120) 5438633..5439550 + 918 WP_064166397.1 alpha/beta hydrolase -
MKK01_RS26125 (MKK01_26125) 5439565..5440506 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
MKK01_RS26130 (MKK01_26130) 5440551..5440988 - 438 WP_012543288.1 D-aminoacyl-tRNA deacylase -
MKK01_RS26135 (MKK01_26135) 5440985..5441845 - 861 WP_008807907.1 virulence factor BrkB family protein -
MKK01_RS26140 (MKK01_26140) 5441839..5442438 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T236792 WP_008807903.1 NZ_CP092632:c5438186-5437803 [Klebsiella variicola subsp. variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T236792 NZ_LR738724:1038930-1039196 [Streptococcus suis]
TTGAGTTATAAGGTTATTTTGTCTGTTAAGGCTCAAAAACAAATTCGAAAGATGGATAAAACTGCTGCAAGTTTGATTTT
GCGGTATCTTTACAAGCATATTGATGGGTGCGACAACCCCAGACAATACGGACAAGCCTTAACTGCCAATCGTTCTGGCC
AATGGCGGTACCGTATCGGCAATTATAGGGTCATCGTCAGCATTGAAGATGATAAATTGATTATAACTGCCCTTGAAGTC
GGGCATCGAAAAGAAGTATATAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT236792 WP_004150355.1 NZ_CP092632:c5438428-5438186 [Klebsiella variicola subsp. variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT236792 NZ_LR738724:1038701-1038940 [Streptococcus suis]
ATGGCAGCTATTACATTGAAAGTTTCTGAGCAGGACAAGCTTTTTATGCAGGCTATGGCAAAGTTTGAAGGCGTGAGTTT
GTCAGAACTCATTCGGACTAAAACCCTTGAAGCCCTTGAAGATGAGTATGATGCGCGTGTGGCAGATATAGCCTGGGCAG
AATACCAAGAAGACTTGGCGAATGGAATTGAGCCGATAACATTAGAACAAATGGCTGAGGAGTTGGGCATTGAGTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References