Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4235399..4236018 Replicon chromosome
Accession NZ_CP092632
Organism Klebsiella variicola subsp. variicola strain ML.9ba2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MKK01_RS20425 Protein ID WP_002892050.1
Coordinates 4235800..4236018 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag MKK01_RS20420 Protein ID WP_008805436.1
Coordinates 4235399..4235773 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKK01_RS20410 (MKK01_20410) 4230554..4231747 + 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MKK01_RS20415 (MKK01_20415) 4231770..4234916 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit AcrB -
MKK01_RS20420 (MKK01_20420) 4235399..4235773 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
MKK01_RS20425 (MKK01_20425) 4235800..4236018 + 219 WP_002892050.1 HHA domain-containing protein Toxin
MKK01_RS20430 (MKK01_20430) 4236177..4236743 + 567 WP_008805435.1 maltose O-acetyltransferase -
MKK01_RS20435 (MKK01_20435) 4236715..4236843 - 129 Protein_4015 hypothetical protein -
MKK01_RS20440 (MKK01_20440) 4236880..4237350 + 471 WP_008805434.1 YlaC family protein -
MKK01_RS20445 (MKK01_20445) 4237319..4238776 - 1458 WP_008805433.1 PLP-dependent aminotransferase family protein -
MKK01_RS20450 (MKK01_20450) 4238877..4239575 + 699 WP_016160391.1 GNAT family protein -
MKK01_RS20455 (MKK01_20455) 4239572..4239712 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MKK01_RS20460 (MKK01_20460) 4239712..4239975 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T236791 WP_002892050.1 NZ_CP092632:4235800-4236018 [Klebsiella variicola subsp. variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T236791 NZ_LR738724:1009416-1010183 [Streptococcus suis]
ATGAGGTTGGAAGAATTTAGTGAGGCTGACTTTCAGAAGGCCTTGCAGCGAACCATTCGGGCTTTAACCCGAGGAAAGAC
GATTCCAGATCAACCGAAAGCTATCTTACTTGGTGGACAAAGCGGAGCAGGTAAGACGACTATTCATCGTATCAAGCAAA
AAGAATTTCAAGGTAATATCATTATCATCGATGGTGACAGCTACCGTTCTCAGCATCCCAATTACTTAGCCCTGCAAGAA
AAGTATGGCAAAGACAGCGTGGACTATACCAAGGGATTTGCAGGAAAAATGGTAGAGCATCTGGTTGATGAACTTAGCAC
ACAAGGTTATCATTTGCTGATTGAAGGGACTTTGCGTACCACTCAAGTTCCTCGTCAGACTGCTCAATTCTTAGCTTCGA
AAGGATACCAAGTTTCTTTAGCTGTCATTGGTACTAAACCAGAGCTTTCTTATCTCAGCACCTTGATTCGTTACGAAGAG
CTTTATGCCATTGATCCCAATCAGGCCAGAGCAACACCAAAAGAACACCATGATGGGATTGTTGAGAACTTGGTTGATAA
CCTAAGGGAGTTAGAAAGAGAGCAACTCTTTGACCAGATTCAGATTTATCAAAGAGACAGGACTTGCATTTATGATTCTG
CAATTGATGAAGGCTCCGCAGCAGAAGTGTTACAAGAGTGTCTCTTTGGAAAATGGAGCAAGGTAGAGGAGGAGATGGTG
AAGGAGAGTGAACTCCTACTTAATGAATTACTTGAGAAAAATAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT236791 WP_008805436.1 NZ_CP092632:4235399-4235773 [Klebsiella variicola subsp. variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT236791 NZ_LR738724:1008940-1009416 [Streptococcus suis]
ATGATTGGAGACAATATCAAGTCGCTACGCCGAACACATGATTTAACACAACCAGAATTTGCGAAAATGGTTGGTATTTC
ACGCAATAGCTTAAGTCGTTATGAAAATGGGACTAGTACAGTTTCAACTGAACTCATCGACCGTATTTGTCAGAAATTTA
ACGTTTCTTATGTCGATATTGTAGGGGAGGATAAGATGTTAACACCTGTTGAAGATTACCAACTGACTTTAAAAGTAGAA
GTGATAAAAGAACGTGGAGCTGCCATTTTATCACAGCTTTATAGATACCAGGATAGTCAAGATATTGCTTTTGATGATGA
ATCCAACCCTTGGATTTTGATGAGTGATGACTTGGCCGAATTGATTAACACAAAAATTTACCTTGTCGAGACTTTCGATG
AAATTGAGCGTTACAATGGCTATTTAGACGGTATTGAGCGGATGTTAGATATGGTACATCATCGGGTGGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References