Detailed information of TA system TA236753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 31322..32033
• Replicon: plasmid pMFDS1011657
• Accession: NZ_CP092628
• Organism: Salmonella enterica subsp. enterica serovar Thompson strain MFDS1011657

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: MJ899_RS23665
• Protein ID: WP_000162415.1
• Coordinates: 31322..31624 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MJ899_RS23670
• Protein ID: WP_000806445.1
• Coordinates: 31695..32033 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ899_RS23635 (MJ899_23630)	26341..26991	-	651	WP_032153356.1	hypothetical protein	-
MJ899_RS23640 (MJ899_23635)	27005..27910	-	906	WP_001258014.1	recombination-associated protein RdgC	-
MJ899_RS23645 (MJ899_23640)	27970..28623	-	654	WP_271232211.1	baseplate protein	-
MJ899_RS23650 (MJ899_23645)	28620..29600	-	981	WP_271232212.1	tail length tape measure protein	-
MJ899_RS23655 (MJ899_23650)	29604..30371	-	768	WP_000203290.1	hypothetical protein	-
MJ899_RS23660 (MJ899_23655)	30368..31159	-	792	WP_000532305.1	hypothetical protein	-
MJ899_RS23665 (MJ899_23660)	31322..31624	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MJ899_RS23670 (MJ899_23665)	31695..32033	+	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
MJ899_RS23675 (MJ899_23670)	32090..32275	+	186	Protein_35	hypothetical protein	-
MJ899_RS23680 (MJ899_23675)	32312..33040	+	729	WP_000986754.1	hypothetical protein	-
MJ899_RS23685 (MJ899_23680)	33074..34267	-	1194	WP_001112633.1	hypothetical protein	-
MJ899_RS23690 (MJ899_23685)	34260..34589	-	330	WP_000542383.1	hypothetical protein	-
MJ899_RS23695 (MJ899_23690)	34918..35571	-	654	WP_000410952.1	hypothetical protein	-
MJ899_RS23700 (MJ899_23695)	35917..36738	+	822	WP_001427910.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92361	92361

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T236753 WP_000162415.1 NZ_CP092628:31322-31624 [Salmonella enterica subsp. enterica serovar Thompson]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T236753 NZ_LR735437:921891-922253 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT236753 WP_000806445.1 NZ_CP092628:31695-32033 [Salmonella enterica subsp. enterica serovar Thompson]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT236753 NZ_LR735437:921724-921894 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3