Detailed information of TA system TA236729

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28969..29238
• Replicon: plasmid p3
• Accession: NZ_CP092592
• Organism: Klebsiella pneumoniae strain EFN 299

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MKT41_RS27510
• Protein ID: WP_001372321.1
• Coordinates: 29113..29238 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	28969..29034 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKT41_RS27480	24679..25206	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
MKT41_RS27485	25264..25497	+	234	WP_000006003.1	DUF905 family protein	-
MKT41_RS27490	25558..27581	+	2024	Protein_35	ParB/RepB/Spo0J family partition protein	-
MKT41_RS27495	27650..28084	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
MKT41_RS27500	28081..28800	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	28812..29036	+	225	NuclAT_0	-	-
-	28812..29036	+	225	NuclAT_0	-	-
-	28812..29036	+	225	NuclAT_0	-	-
-	28812..29036	+	225	NuclAT_0	-	-
-	28969..29034	-	66	-	-	Antitoxin
MKT41_RS27505	29022..29171	+	150	Protein_38	plasmid maintenance protein Mok	-
MKT41_RS27510	29113..29238	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MKT41_RS27515	29557..29853	-	297	Protein_40	hypothetical protein	-
MKT41_RS27520	30153..30449	+	297	WP_001272251.1	hypothetical protein	-
MKT41_RS27525	30560..31381	+	822	WP_001234445.1	DUF932 domain-containing protein	-
MKT41_RS27530	31678..32268	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
MKT41_RS27535	32603..32986	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MKT41_RS27540	33180..33851	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
MKT41_RS27545	33988..34215	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T236729 WP_001372321.1 NZ_CP092592:29113-29238 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T236729 NZ_LR699570:3520488-3520832 [Mycobacterium tuberculosis variant bovis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT236729 NZ_CP092592:c29034-28969 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin