Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 618321..619120 Replicon chromosome
Accession NZ_CP092533
Organism Escherichia coli strain DS566-1

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag MKD47_RS02990 Protein ID WP_000347275.1
Coordinates 618321..618785 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MKD47_RS02995 Protein ID WP_001307405.1
Coordinates 618785..619120 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD47_RS02960 (613322) 613322..613756 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MKD47_RS02965 (613774) 613774..614652 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MKD47_RS02970 (614642) 614642..615421 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
MKD47_RS02975 (615432) 615432..615905 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
MKD47_RS02980 (615928) 615928..617208 - 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
MKD47_RS02985 (617457) 617457..618266 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MKD47_RS02990 (618321) 618321..618785 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MKD47_RS02995 (618785) 618785..619120 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MKD47_RS03000 (619269) 619269..620840 - 1572 WP_001273752.1 galactarate dehydratase -
MKD47_RS03005 (621215) 621215..622549 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MKD47_RS03010 (622565) 622565..623335 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T236396 WP_000347275.1 NZ_CP092533:c618785-618321 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T236396 NZ_LR590480:c2170085-2169819 [Bordetella parapertussis]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGTGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATACCCGCCAGGTCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT236396 WP_001307405.1 NZ_CP092533:c619120-618785 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT236396 NZ_LR590480:c2169832-2169554 [Bordetella parapertussis]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCGATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGG
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References