Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeV-yafW/CbtA-CbeA
Location 4593465..4594258 Replicon chromosome
Accession NZ_CP092493
Organism Citrobacter freundii strain SCLZS47

Toxin (Protein)


Gene name yeeV Uniprot ID S1EZ92
Locus tag MJ253_RS22770 Protein ID WP_000854726.1
Coordinates 4593881..4594258 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EBQ7
Locus tag MJ253_RS22765 Protein ID WP_001548930.1
Coordinates 4593465..4593791 (+) Length 109 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ253_RS22720 (4588769) 4588769..4589590 + 822 WP_008324532.1 DUF932 domain-containing protein -
MJ253_RS22725 (4589808) 4589808..4590509 + 702 WP_008324530.1 WYL domain-containing protein -
MJ253_RS22730 (4590556) 4590556..4590792 + 237 WP_001115854.1 protein YpjK -
MJ253_RS22735 (4590792) 4590792..4591235 + 444 WP_053263801.1 lipoprotein YafY -
MJ253_RS22740 (4591258) 4591258..4591725 + 468 WP_001547765.1 protein YkfB -
MJ253_RS22745 (4591802) 4591802..4592056 + 255 WP_101743273.1 DUF905 domain-containing protein -
MJ253_RS27165 (4592070) 4592070..4592168 + 99 WP_232051968.1 DUF905 family protein -
MJ253_RS22750 (4592266) 4592266..4592724 + 459 WP_000211838.1 antirestriction protein -
MJ253_RS22755 (4592740) 4592740..4593216 + 477 WP_016243826.1 RadC family protein -
MJ253_RS22760 (4593225) 4593225..4593446 + 222 WP_016239820.1 DUF987 domain-containing protein -
MJ253_RS22765 (4593465) 4593465..4593791 + 327 WP_001548930.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
MJ253_RS22770 (4593881) 4593881..4594258 + 378 WP_000854726.1 TA system toxin CbtA family protein Toxin
MJ253_RS22775 (4594255) 4594255..4594542 + 288 Protein_4474 DUF5983 family protein -
MJ253_RS22780 (4594635) 4594635..4595863 + 1229 WP_148116659.1 IS3-like element ISEc27 family transposase -
MJ253_RS22785 (4595873) 4595873..4596079 + 207 Protein_4476 DUF5983 family protein -
MJ253_RS22790 (4596091) 4596091..4596288 + 198 WP_000839269.1 DUF957 domain-containing protein -
MJ253_RS22795 (4596373) 4596373..4597215 + 843 WP_001548928.1 DUF4942 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(6-103)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14164.10 Da        Isoelectric Point: 7.8045

>T236296 WP_000854726.1 NZ_CP092493:4593881-4594258 [Citrobacter freundii]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Download         Length: 378 bp

>T236296 NZ_LR217818:c3395616-3395365 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12301.97 Da        Isoelectric Point: 7.0273

>AT236296 WP_001548930.1 NZ_CP092493:4593465-4593791 [Citrobacter freundii]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Download         Length: 327 bp

>AT236296 NZ_LR217818:c3395368-3395114 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1EZ92


Antitoxin

Source ID Structure
AlphaFold DB S1EBQ7

References