Detailed information of TA system TA236277

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 76193..76875
• Replicon: chromosome
• Accession: NZ_CP092493
• Organism: Citrobacter freundii strain SCLZS47

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: MJ253_RS00370
• Protein ID: WP_108168483.1
• Coordinates: 76193..76534 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: MJ253_RS00375
• Protein ID: WP_108168484.1
• Coordinates: 76555..76875 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ253_RS00350 (72871)	72871..73398	-	528	WP_134216361.1	hypothetical protein	-
MJ253_RS00355 (73445)	73445..74104	-	660	WP_134216360.1	antiviral reverse transcriptase Drt3a	-
MJ253_RS00360 (74128)	74128..74754	-	627	WP_134216359.1	SLATT domain-containing protein	-
MJ253_RS00365 (75246)	75246..76079	-	834	WP_099530882.1	DUF4942 domain-containing protein	-
MJ253_RS00370 (76193)	76193..76534	-	342	WP_108168483.1	TA system toxin CbtA family protein	Toxin
MJ253_RS00375 (76555)	76555..76875	-	321	WP_108168484.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MJ253_RS00380 (76894)	76894..77115	-	222	WP_108168485.1	DUF987 domain-containing protein	-
MJ253_RS00385 (77125)	77125..77601	-	477	WP_108168486.1	RadC family protein	-
MJ253_RS00390 (77617)	77617..78075	-	459	WP_108168487.1	antirestriction protein	-
MJ253_RS00395 (78156)	78156..78392	-	237	WP_108168488.1	DUF905 domain-containing protein	-
MJ253_RS00400 (78470)	78470..78880	-	411	WP_043082399.1	hypothetical protein	-
MJ253_RS00405 (78966)	78966..79580	-	615	WP_108168489.1	hypothetical protein	-
MJ253_RS00410 (79577)	79577..80281	-	705	WP_108168490.1	WYL domain-containing protein	-
MJ253_RS00415 (80482)	80482..81315	-	834	WP_108168491.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	69697..87852	18155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-105)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12956.85 Da
• Isoelectric Point: 7.2015

>T236277 WP_108168483.1 NZ_CP092493:c76534-76193 [Citrobacter freundii]
MKPQPATTSRAAELYLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHIDAGIMLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNAVR

Nucleotide

• Length: 342 bp

>T236277 NZ_LR217818:747921-748217 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12027.71 Da
• Isoelectric Point: 6.9370

>AT236277 WP_108168484.1 NZ_CP092493:c76875-76555 [Citrobacter freundii]
MRSNITWGLQRDITPCMGARLVQEGNRLHYLADRASITGKFSDDECLKLDVVFPHFISRIESMLTTGELNPRHAHCITIY
HNGFTCEADTLGSCAYVYVVVYSTQR

Nucleotide

• Length: 321 bp

>AT236277 NZ_LR217818:748219-748614 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA