Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3390117..3390735 Replicon chromosome
Accession NZ_CP092452
Organism Escherichia coli isolate TKEC21-59

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MJJ11_RS16550 Protein ID WP_001291435.1
Coordinates 3390517..3390735 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MJJ11_RS16545 Protein ID WP_000344800.1
Coordinates 3390117..3390491 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJJ11_RS16535 (3385206) 3385206..3386399 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MJJ11_RS16540 (3386422) 3386422..3389571 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
MJJ11_RS16545 (3390117) 3390117..3390491 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MJJ11_RS16550 (3390517) 3390517..3390735 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MJJ11_RS16555 (3390907) 3390907..3391458 + 552 WP_000102564.1 maltose O-acetyltransferase -
MJJ11_RS16560 (3391574) 3391574..3392044 + 471 WP_000136192.1 YlaC family protein -
MJJ11_RS16565 (3392208) 3392208..3392684 + 477 Protein_3258 cyclic-guanylate-specific phosphodiesterase PdeB -
MJJ11_RS16570 (3392749) 3392749..3393911 + 1163 WP_085947771.1 IS3-like element IS3 family transposase -
MJJ11_RS16575 (3394014) 3394014..3394711 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
MJJ11_RS16580 (3394646) 3394646..3395497 - 852 WP_239812901.1 integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T236166 WP_001291435.1 NZ_CP092452:3390517-3390735 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T236166 NZ_LR135443:2268548-2268814 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT236166 WP_000344800.1 NZ_CP092452:3390117-3390491 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT236166 NZ_LR135443:2268285-2268548 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References