Detailed information of TA system TA236165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3018811..3019516
• Replicon: chromosome
• Accession: NZ_CP092452
• Organism: Escherichia coli isolate TKEC21-59

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: MJJ11_RS14705
• Protein ID: WP_000539521.1
• Coordinates: 3018811..3019197 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MJJ11_RS14710
• Protein ID: WP_001280945.1
• Coordinates: 3019187..3019516 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJJ11_RS14685 (3014815)	3014815..3015441	+	627	WP_001295292.1	glutathione S-transferase GstB	-
MJJ11_RS14690 (3015438)	3015438..3016553	-	1116	WP_000555031.1	aldose sugar dehydrogenase YliI	-
MJJ11_RS14695 (3016664)	3016664..3017047	-	384	WP_000497137.1	biofilm formation regulator BssR	-
MJJ11_RS14700 (3017260)	3017260..3018585	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
MJJ11_RS14705 (3018811)	3018811..3019197	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MJJ11_RS14710 (3019187)	3019187..3019516	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
MJJ11_RS14715 (3019586)	3019586..3020914	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
MJJ11_RS14720 (3020922)	3020922..3023270	-	2349	WP_085448431.1	EAL domain-containing protein	-
MJJ11_RS14725 (3023448)	3023448..3024359	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T236165 WP_000539521.1 NZ_CP092452:3018811-3019197 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T236165 NZ_LR135437:12158-12415 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT236165 WP_001280945.1 NZ_CP092452:3019187-3019516 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT236165 NZ_LR135437:11896-12165 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6