Detailed information of TA system TA236095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 475918..476555
• Replicon: chromosome
• Accession: NZ_CP092439
• Organism: Bacillus velezensis strain 504

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: MG974_RS02475
• Protein ID: WP_003156187.1
• Coordinates: 476205..476555 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: MG974_RS02470
• Protein ID: WP_003156188.1
• Coordinates: 475918..476199 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MG974_RS02450 (MG974_02450)	472283..472882	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
MG974_RS02455 (MG974_02455)	472975..473340	+	366	WP_060657770.1	holo-ACP synthase	-
MG974_RS02460 (MG974_02460)	473505..474512	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
MG974_RS02465 (MG974_02465)	474629..475798	+	1170	WP_060657768.1	alanine racemase	-
MG974_RS02470 (MG974_02470)	475918..476199	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
MG974_RS02475 (MG974_02475)	476205..476555	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
MG974_RS02480 (MG974_02480)	476673..477494	+	822	WP_014304404.1	STAS domain-containing protein	-
MG974_RS02485 (MG974_02485)	477499..477864	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
MG974_RS02490 (MG974_02490)	477867..478268	+	402	WP_003156178.1	anti-sigma regulatory factor	-
MG974_RS02495 (MG974_02495)	478280..479287	+	1008	WP_025853725.1	PP2C family protein-serine/threonine phosphatase	-
MG974_RS02500 (MG974_02500)	479351..479680	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
MG974_RS02505 (MG974_02505)	479677..480159	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
MG974_RS02510 (MG974_02510)	480125..480913	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
MG974_RS02515 (MG974_02515)	480913..481515	+	603	WP_003156170.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T236095 WP_003156187.1 NZ_CP092439:476205-476555 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T236095 NZ_LR135310:31793-32050 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT236095 WP_003156188.1 NZ_CP092439:475918-476199 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT236095 NZ_LR135310:31531-31800 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5