Detailed information of TA system TA235956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1703454..1704035
• Replicon: chromosome
• Accession: NZ_CP092360
• Organism: Wielerella bovis strain 07LB258

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: MIS31_RS08825
• Protein ID: WP_249446465.1
• Coordinates: 1703454..1703783 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: MIS31_RS08830
• Protein ID: WP_249446466.1
• Coordinates: 1703790..1704035 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MIS31_RS08810 (MIS31_08810)	1698517..1699950	+	1434	WP_249446462.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
MIS31_RS08815 (MIS31_08815)	1700089..1701102	+	1014	WP_249446463.1	CAP domain-containing protein	-
MIS31_RS08820 (MIS31_08820)	1701561..1703009	-	1449	WP_249446464.1	metalloprotease TldD	-
MIS31_RS08825 (MIS31_08825)	1703454..1703783	-	330	WP_249446465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MIS31_RS08830 (MIS31_08830)	1703790..1704035	-	246	WP_249446466.1	antitoxin	Antitoxin
MIS31_RS08835 (MIS31_08835)	1704237..1704623	-	387	WP_249446467.1	helix-turn-helix transcriptional regulator	-
MIS31_RS08840 (MIS31_08840)	1704746..1705300	+	555	WP_249446468.1	hypothetical protein	-
MIS31_RS08845 (MIS31_08845)	1705373..1708039	-	2667	WP_249446469.1	aminopeptidase N	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-107)

Antitoxin

• Antitoxin PemI-like (4-81)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12142.03 Da
• Isoelectric Point: 10.1784

>T235956 WP_249446465.1 NZ_CP092360:c1703783-1703454 [Wielerella bovis]
MERGDIFLVNLDPTTGHEQQGTRPVLIISPKAFNALTQVPIVLPITTKSQFARTQGFAVHLDERTKTQGIVRTDQSRALD
IQARNGRKLETLPIDLMNEVLAKMATFLS

Nucleotide

• Length: 330 bp

>T235956 NZ_LR134515:c1581769-1581398 [Actinobacillus pleuropneumoniae]
ATGTATGAAATAGTCTTTTATCGAGATAAGCGGGGGAGAGAACCCGTAAAAGAATTTCTGTTAAGGCTACTAAAAGAACG
GCAGGAAGGCAGTCGAGAACGATTACACAAAATCAGCCATCATTTGAGTATTTTACATTTGCACGGTACTCGAGCCGGTG
AGAATTATATTAAGCATTTGGAAGATCGTATTTGGCAGTTACGTCCGGTGGGCGATTGCCTGTTATTTGCAAGTATTATT
CGGGGAAAATTTGTGTTGTTGCATTATTTTGCCAAACAGAATTATCGAATTCCGAAACGAGAAATAGAACGTGCAAAAGT
TCGGTTGGCGGATTTACAGGAGAGAATAAAAGATGAGCCCTATTGGTTCTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8921.22 Da
• Isoelectric Point: 4.9189

>AT235956 WP_249446466.1 NZ_CP092360:c1704035-1703790 [Wielerella bovis]
MYTVSLRKVGGSTMLAIPPALLQSLDLGKQVHLHTENGRLIIEPARPQYTLAELLAQCDPNAPRDVESQTWLDAPAVGQE
W

Nucleotide

• Length: 246 bp

>AT235956 NZ_LR134515:c1581417-1581124 [Actinobacillus pleuropneumoniae]
ATGAGCCCTATTGGTTCTAGTTGGACATCGTTTGAACAAGAAATTTTTGACGAGTCGGAAATAGCGGAAACTCAGTTTCG
CATACGACTTATTTTAGAAATCATTGAAACCCGTCAGCAGCTCGGTATTTCTCAACGAAAATTGGAGAAATTAAGCGGCG
TAAAACAATCTATGATTGCTCGGGTAGAAAAAGGCTCGGTTAATCCGTCGTTGGCGACGGTATTAAAGTTGCTTGTGCCG
TTGGGGAAAACTTTACAAATCGTACCGTTAGATAAAAACAAAACGGTACGTTAA