Detailed information of TA system TA235940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 928284..929074
• Replicon: plasmid pRGM101b
• Accession: NZ_CP092346
• Organism: Rhizobium gallicum strain M101

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: L2W42_RS28140
• Protein ID: WP_194730543.1
• Coordinates: 928577..929074 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: L2W42_RS28135
• Protein ID: WP_194730542.1
• Coordinates: 928284..928580 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2W42_RS28105 (L2W42_28105)	923951..924967	-	1017	WP_239602910.1	taurine ABC transporter substrate-binding protein	-
L2W42_RS28110 (L2W42_28110)	925234..925719	+	486	WP_239602913.1	AAA family ATPase	-
L2W42_RS28115 (L2W42_28115)	925899..926111	+	213	WP_194730540.1	hypothetical protein	-
L2W42_RS28120 (L2W42_28120)	926162..926746	-	585	WP_239602916.1	hypothetical protein	-
L2W42_RS28125 (L2W42_28125)	926729..927127	+	399	WP_239602919.1	hypothetical protein	-
L2W42_RS28130 (L2W42_28130)	927280..927754	-	475	Protein_915	VOC family protein	-
L2W42_RS28135 (L2W42_28135)	928284..928580	+	297	WP_194730542.1	DUF1778 domain-containing protein	Antitoxin
L2W42_RS28140 (L2W42_28140)	928577..929074	+	498	WP_194730543.1	GNAT family N-acetyltransferase	Toxin
L2W42_RS28145 (L2W42_28145)	929198..929581	-	384	WP_239602921.1	type II toxin-antitoxin system VapC family toxin	-
L2W42_RS28150 (L2W42_28150)	929578..929777	-	200	Protein_919	type II toxin-antitoxin system VapB family antitoxin	-
L2W42_RS28155 (L2W42_28155)	930278..930721	+	444	WP_239602923.1	acetyl-CoA carboxylase biotin carboxyl carrier protein subunit	-
L2W42_RS28160 (L2W42_28160)	930721..932112	+	1392	WP_239602925.1	acetyl-CoA carboxylase biotin carboxylase subunit	-
L2W42_RS28165 (L2W42_28165)	932109..932537	+	429	WP_239602926.1	acetyl-CoA carboxylase	-
L2W42_RS28170 (L2W42_28170)	932534..933241	+	708	WP_239602929.1	5-oxoprolinase subunit PxpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..2542150	2542150

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-97)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 17809.51 Da
• Isoelectric Point: 9.0370

>T235940 WP_194730543.1 NZ_CP092346:928577-929074 [Rhizobium gallicum]
VTLSAPTPLADHHELAEFISGVVELDDWLRRRARANQAGGASRTFVVCEGNRVIAYYALASGAVKQPEAPGRFRRNMPDP
IPVAVLGRLAIDRTFQGRGLGRALVRDAGLRLLNAAEILGIRGVLVHAISDDARAFYEAVGFLPSPSDPMMLMVGLRDLN
NALNP

Nucleotide

• Length: 498 bp

>T235940 NZ_LR134495:c204641-204372 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCCTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGTGATCACGCTTTAACAGGTGAATGGAAAG
GCTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGGAAAGTGGAAGATGATAAGTTAGAACTACTACGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11070.61 Da
• Isoelectric Point: 7.0159

>AT235940 WP_194730542.1 NZ_CP092346:928284-928580 [Rhizobium gallicum]
MPTSHTSKSADASVPLNIRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTIFTVSPELYAEYLARLDAPAQ
PNERLKRTMTTKAPWDEA

Nucleotide

• Length: 297 bp

>AT235940 NZ_LR134495:c204894-204628 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTATCTGATGTGATGCGAATGACATTAGCAAAAATTGCGACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA