Detailed information of TA system TA023563

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4706927..4707339
• Replicon: chromosome
• Accession: NC_011993
• Organism: Escherichia coli LF82

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: LF82_RS23205
• Protein ID: WP_000132614.1
• Coordinates: 4706927..4707268 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4707263..4707339 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LF82_RS23185	4701996..4703276	-	1281	WP_014640274.1	DUF445 domain-containing protein	-
LF82_RS23190	4703461..4704381	+	921	WP_000181192.1	Rpn family recombination-promoting nuclease/putative transposase	-
LF82_RS25450	4704609..4704689	+	81	WP_020233658.1	hypothetical protein	-
LF82_RS23605	4704787..4706880	+	2094	WP_000648245.1	DUF262 domain-containing protein	-
LF82_RS23205	4706927..4707268	-	342	WP_000132614.1	endoribonuclease SymE	Toxin
-	4707263..4707339	+	77	NuclAT_6	-	Antitoxin
-	4707263..4707339	+	77	NuclAT_6	-	Antitoxin
-	4707263..4707339	+	77	NuclAT_6	-	Antitoxin
-	4707263..4707339	+	77	NuclAT_6	-	Antitoxin
-	4707263..4707339	+	77	NuclAT_7	-	Antitoxin
-	4707263..4707339	+	77	NuclAT_7	-	Antitoxin
-	4707263..4707339	+	77	NuclAT_7	-	Antitoxin
-	4707263..4707339	+	77	NuclAT_7	-	Antitoxin
LF82_RS23210	4707489..4709195	-	1707	WP_001100193.1	restriction endonuclease subunit S	-
LF82_RS23215	4709195..4710664	-	1470	WP_001540814.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T23563 WP_000132614.1 NC_011993:c4707268-4706927 [Escherichia coli LF82]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T23563 NC_011993:c4707268-4706927 [Escherichia coli LF82]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT23563 NC_011993:4707263-4707339 [Escherichia coli LF82]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin