Detailed information of TA system TA235628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3555537..3556211
• Replicon: chromosome
• Accession: NZ_CP092065
• Organism: Edwardsiella ictaluri strain S15-163

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MAY82_RS17320
• Protein ID: WP_233420345.1
• Coordinates: 3555834..3556211 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MAY82_RS17315
• Protein ID: WP_278068344.1
• Coordinates: 3555537..3555818 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAY82_RS17285 (MAY82_17185)	3551457..3551804	+	348	WP_015871339.1	hypothetical protein	-
MAY82_RS17290 (MAY82_17190)	3551914..3552558	+	645	WP_015871337.1	DUF1367 family protein	-
MAY82_RS17295 (MAY82_17195)	3552561..3552860	+	300	WP_015871336.1	DUF1364 family protein	-
MAY82_RS17300 (MAY82_17200)	3553005..3554045	+	1041	WP_015871335.1	hypothetical protein	-
MAY82_RS17305 (MAY82_17205)	3554046..3554555	+	510	WP_071525693.1	hypothetical protein	-
MAY82_RS17310 (MAY82_17210)	3554922..3555434	-	513	WP_015871334.1	DUF2442 domain-containing protein	-
MAY82_RS17315 (MAY82_17215)	3555537..3555818	-	282	WP_278068344.1	helix-turn-helix transcriptional regulator	Antitoxin
MAY82_RS17320 (MAY82_17220)	3555834..3556211	-	378	WP_233420345.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MAY82_RS17325 (MAY82_17225)	3556612..3557394	+	783	WP_015871331.1	Rha family transcriptional regulator	-
MAY82_RS17330 (MAY82_17230)	3557626..3557922	+	297	WP_278174896.1	hypothetical protein	-
MAY82_RS17335 (MAY82_17235)	3558118..3558936	+	819	WP_015871329.1	hypothetical protein	-
MAY82_RS17340 (MAY82_17240)	3559042..3559286	+	245	Protein_3343	transposase	-
MAY82_RS17345 (MAY82_17245)	3559418..3559648	+	231	WP_015871327.1	ParD-like family protein	-
MAY82_RS17350 (MAY82_17250)	3559623..3559952	+	330	WP_015871326.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MAY82_RS17355 (MAY82_17255)	3560036..3560383	+	348	WP_035608895.1	phage holin, lambda family	-
MAY82_RS17360 (MAY82_17260)	3560386..3560937	+	552	WP_015871324.1	putative peptidoglycan-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3531121..3595196	64075

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (32-119)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-85)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14680.05 Da
• Isoelectric Point: 10.1221

>T235628 WP_233420345.1 NZ_CP092065:c3556211-3555834 [Edwardsiella ictaluri]
MGKNLRKHNNIVMFHSMNYTIEYYSEEVRLEVDQLPMGMRVRYQHLVERMEIYGSNLGEPHTSPFGDGLFELRIKGSDGI
ARVFYCTLTGKRIVMLHSFIKKTQKTPSAERKKAETRMKEVKHGW

Nucleotide

• Length: 378 bp

>T235628 NZ_LR134312:2624576-2624719 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10267.73 Da
• Isoelectric Point: 6.4690

>AT235628 WP_278068344.1 NZ_CP092065:c3555818-3555537 [Edwardsiella ictaluri]
MTHDEMAAKWMEDPAFKAEYDAIEEEYALLDEMLAARKNAGLTQAQVAERMGTKATAITRMESNLASGQSGPSFATLKKF
AHATGKKLQIRFV

Nucleotide

• Length: 282 bp

>AT235628 NZ_LR134312:c2624476-2624278 [Enterococcus faecalis]
TTTTTAGGTAATTGTGCTATAATGGTAATGAAAAGAGAGCTATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTAAGA
CGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATT
TCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA