Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 576688..577280 Replicon chromosome
Accession NZ_CP092065
Organism Edwardsiella ictaluri strain S15-163

Toxin (Protein)


Gene name Hha Uniprot ID D0ZEC2
Locus tag MAY82_RS02875 Protein ID WP_012847872.1
Coordinates 577077..577280 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID C5BCY7
Locus tag MAY82_RS02870 Protein ID WP_015870487.1
Coordinates 576688..577056 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAY82_RS02860 (MAY82_02845) 571948..573132 + 1185 WP_015870489.1 efflux RND transporter periplasmic adaptor subunit -
MAY82_RS02865 (MAY82_02850) 573176..576328 + 3153 WP_015870488.1 efflux RND transporter permease subunit -
MAY82_RS02870 (MAY82_02855) 576688..577056 + 369 WP_015870487.1 Hha toxicity modulator TomB Antitoxin
MAY82_RS02875 (MAY82_02860) 577077..577280 + 204 WP_012847872.1 HHA domain-containing protein Toxin
MAY82_RS02880 (MAY82_02865) 577300..577407 - 108 Protein_549 IS5/IS1182 family transposase -
MAY82_RS02890 (MAY82_02875) 578385..578714 + 330 WP_015870485.1 MGMT family protein -
MAY82_RS02895 (MAY82_02880) 578760..579626 + 867 WP_015870484.1 acyl-CoA thioesterase II -
MAY82_RS02900 (MAY82_02885) 579660..580941 - 1282 Protein_552 ammonium transporter AmtB -
MAY82_RS02905 (MAY82_02890) 580963..581301 - 339 WP_015870482.1 P-II family nitrogen regulator -
MAY82_RS02910 (MAY82_02895) 581404..581571 + 168 WP_015870481.1 hypothetical protein -
MAY82_RS02915 (MAY82_02900) 581600..582061 - 462 WP_015870480.1 Lrp/AsnC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8103.37 Da        Isoelectric Point: 5.0914

>T235624 WP_012847872.1 NZ_CP092065:577077-577280 [Edwardsiella ictaluri]
MTKIDYLMKLRKCTTLDTLERVIEKNKYELSDDELEIFYSAADHRLAELTMNKLYDKIPAEVWQYVR

Download         Length: 204 bp

>T235624 NZ_LR134312:c2618661-2618320 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14287.03 Da        Isoelectric Point: 5.4786

>AT235624 WP_015870487.1 NZ_CP092065:576688-577056 [Edwardsiella ictaluri]
MDENTSYQHDISELKYLCDYLYHQGIDVLGESNHGWVSDPTAEVNLQLNELIEHIASIAQSFKIKYPRHSDLAEMLDYYL
DETYALFGTYSISETALRQWLRTKRRMAYCLAHEKRNAALHV

Download         Length: 369 bp

>AT235624 NZ_LR134312:c2618891-2618661 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A034SMG4

Antitoxin

Source ID Structure
AlphaFold DB C5BCY7

References