Detailed information of TA system TA235622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3562678..3563353
• Replicon: chromosome
• Accession: NZ_CP092062
• Organism: Edwardsiella ictaluri strain S00-1912

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MAY76_RS17275
• Protein ID: WP_233420345.1
• Coordinates: 3562976..3563353 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: C5BBM8
• Locus tag: MAY76_RS17270
• Protein ID: WP_015871333.1
• Coordinates: 3562678..3562986 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAY76_RS17240 (MAY76_17105)	3558598..3558945	+	348	WP_015871339.1	hypothetical protein	-
MAY76_RS17245 (MAY76_17110)	3559055..3559699	+	645	WP_015871337.1	DUF1367 family protein	-
MAY76_RS17250 (MAY76_17115)	3559702..3560001	+	300	WP_015871336.1	DUF1364 family protein	-
MAY76_RS17255 (MAY76_17120)	3560146..3561186	+	1041	WP_015871335.1	hypothetical protein	-
MAY76_RS17260 (MAY76_17125)	3561187..3561696	+	510	WP_071525693.1	hypothetical protein	-
MAY76_RS17265 (MAY76_17130)	3562063..3562575	-	513	WP_015871334.1	DUF2442 domain-containing protein	-
MAY76_RS17270 (MAY76_17135)	3562678..3562986	-	309	WP_015871333.1	helix-turn-helix transcriptional regulator	Antitoxin
MAY76_RS17275 (MAY76_17140)	3562976..3563353	-	378	WP_233420345.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MAY76_RS17280 (MAY76_17145)	3563754..3564536	+	783	WP_015871331.1	Rha family transcriptional regulator	-
MAY76_RS17285 (MAY76_17150)	3564658..3565173	+	516	WP_034165088.1	hypothetical protein	-
MAY76_RS17290 (MAY76_17155)	3565262..3565900	+	639	WP_278068662.1	hypothetical protein	-
MAY76_RS17295 (MAY76_17160)	3565897..3566079	+	183	WP_278068663.1	hypothetical protein	-
MAY76_RS17300 (MAY76_17165)	3566185..3566429	+	245	Protein_3335	transposase	-
MAY76_RS17305 (MAY76_17170)	3566561..3566791	+	231	WP_015871327.1	ParD-like family protein	-
MAY76_RS17310 (MAY76_17175)	3566766..3567095	+	330	WP_015871326.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MAY76_RS17315 (MAY76_17180)	3567179..3567527	+	349	Protein_3338	phage holin, lambda family	-
MAY76_RS17320 (MAY76_17185)	3567530..3568081	+	552	WP_015871324.1	putative peptidoglycan-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3538257..3598479	60222

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (32-119)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14680.05 Da
• Isoelectric Point: 10.1221

>T235622 WP_233420345.1 NZ_CP092062:c3563353-3562976 [Edwardsiella ictaluri]
MGKNLRKHNNIVMFHSMNYTIEYYSEEVRLEVDQLPMGMRVRYQHLVERMEIYGSNLGEPHTSPFGDGLFELRIKGSDGI
ARVFYCTLTGKRIVMLHSFIKKTQKTPSAERKKAETRMKEVKHGW

Nucleotide

• Length: 378 bp

>T235622 NZ_LR134312:c2546134-2545982 [Enterococcus faecalis]
GTGGTGCGTATGAGCGTACTTCCAAAATTTACGGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCT
AGGTTTTGGTATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11207.86 Da
• Isoelectric Point: 8.5371

>AT235622 WP_015871333.1 NZ_CP092062:c3562986-3562678 [Edwardsiella ictaluri]
MAGKRTPPTMTHDEMAAKWMEDPAFKAEYDAIEEEYALLDEMLAARKNAGLTQAQVAERMGTKATAITRMESNLASGQSG
PSFATLKKFAHATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT235622 NZ_LR134312:2545862-2546049 [Enterococcus faecalis]
TGTGCTATAATGAAAATGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGCAGAGCCGTTTAAGACGGTGACTTTTT
GAATTATTTAAAAATAACCGTACTCGGTCAAAGTTGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGT
GCAATCAAAGCAATGGTAAACATACCAA

Structures

Antitoxin

• AlphaFold DB: C5BBM8